Lyophilization Method
Lyophilization Method - Related Videos
11:14
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
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Cited by 19 •
2015
Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
07:27
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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Cited by 7 •
2016
We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.
02:31
Extracellular Matrix-derived Foam Fabrication via Lyophilization: A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues
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2023
This video describes the method for synthesizing pure ECM-derived foams without the need for chemical crosslinking. These ECM-derived foams find applications as advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
10:36
Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting
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Cited by 9 •
2022
This article describes protocols for high-throughput gelatin methacryloyl microgel fabrication using microfluidic devices, converting microgels to resuspendable powder (micro-aerogels), the chemical assembly of microgels to form granular hydrogel scaffolds, and developing granular hydrogel bioinks with preserved microporosity for 3D bioprinting.
07:06
Particle Agglutination Method for Poliovirus Identification
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2011
A recently developed novel particle agglutination (PA) assay utilizing virus receptor molecule allowed a rapid and easy identification of poliovirus (PV). In this article, we will show the procedure for the PA assay for PV identification.
FAQs
Reduced pressure changes the conditions enough for frozen water to leave as vapor through sublimation rather than pass through a liquid state. This phase transition helps remove ice while the material remains frozen. In bioengineering, this distinction matters because preserving the frozen matrix can support retention of structure and stability during drying.
The phases remove different forms of water. During primary drying, ice undergoes sublimation under reduced pressure; secondary drying then removes moisture that remains bound to the material. Separating these functions allows the process to address both frozen water and residual moisture, supporting a final product intended for storage and later rehydration.
A porous dried matrix is important because it creates a product that can be rehydrated after storage. The structure also reflects the preservation goal: remove water without losing the material’s organization. For bioengineered proteins, cells, enzymes, vaccines, biomaterials, and formulations, this outcome can support handling and subsequent use.
Liquid conditions can promote degradation for some biological products, whereas drying reduces the water present during storage. The resulting solid form can extend shelf life and simplify transport, making the approach useful when proteins, vaccines, enzymes, cells, biomaterials, or pharmaceutical formulations need greater storage stability.
Applications include proteins, vaccines, enzymes, cells, biomaterials, and pharmaceutical formulations. These materials may be vulnerable to degradation while stored in liquid conditions, so converting them into a dry product can support longer storage and easier transport. The method therefore applies across biological materials and engineered formulations rather than to one product class.
It contributes by producing a dry, porous material that can be stored and later rehydrated. This combination can extend shelf life, simplify transport, and help maintain structure and stability. In bioengineering, those outcomes support the development of stable products ranging from biological reagents and vaccines to biomaterials and pharmaceutical formulations.