Ribosomal Rna
Ribosomal Rna - Related Videos
02:53
Ribosomal RNA Synthesis
0 Views •
2020
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells. Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
02:53
Ribosomal RNA Synthesis
0 Views •
2023
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells. Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
06:21
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
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Cited by 20 •
2013
A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.
13:16
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
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Cited by 26 •
2018
Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Cited by 119 •
2014
Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.
FAQs
Within the ribosome, rRNA provides the organized framework that brings messenger RNA and transfer RNAs into the correct translational arrangement. This positioning aligns codons with anticodons while the RNA-based catalytic center supports peptide-bond formation. For bioengineering, that coordinated architecture links sequence-level changes to possible effects on translation performance and protein output.
Conserved rRNA sequences provide molecular features that can be compared across organisms. Their persistence supports evolutionary studies, while sequence-based analysis can help distinguish microbial groups for identification. This makes rRNA valuable beyond its translational role: the same class of molecules can provide information about biological relationships and microbial classification.
Changing rRNA can be used to explore whether ribosomes acquire altered specificity, efficiency, or resistance to inhibitors. These properties describe different engineering outcomes: specificity concerns what translation system components are recognized, efficiency concerns performance, and inhibitor resistance concerns continued function under inhibitory conditions. Such modifications connect ribosome structure with controllable translation behavior.
Researchers can examine conserved rRNA sequences as identifying molecular information when studying microbial samples. Comparisons of these sequences can contribute to distinguishing organisms and placing them in an evolutionary context. The approach is especially useful when the research question requires both microbial classification and interpretation of relationships among the organisms being examined.
In recombinant protein production, rRNA is relevant because it forms part of the translation machinery that converts genetic information into protein. Engineering attention to this component can support efforts to optimize translation systems and improve protein output. The resulting focus is not only on the introduced gene, but also on the ribosomal environment that expresses it.
Synthetic biological circuits depend on controlled expression of genetic information, so their performance is connected to the translation machinery that produces encoded proteins. rRNA provides a bioengineering target for investigating how altered ribosome specificity or efficiency might affect circuit behavior. This context makes ribosome design relevant to constructing systems with more deliberately tuned protein production.