Shaking Water Bath

A shaking water bath is a laboratory instrument that combines controlled-temperature water heating with continuous agitation to treat, mix, or incubate samples under uniform conditions. Samples placed in sealed vessels or containers are immersed in a thermostatically regulated water bath while a platform moves at a set speed, promoting heat transfer and reducing temperature gradients through gentle mixing. In bioengineering, shaking water baths support cell and tissue culture, enzyme reactions, solubilization, and preparation of biological solutions where consistent temperature and suspension are important. Their controlled thermal and mechanical environment improves experimental reproducibility and helps researchers study processes sensitive to temperature or mixing.

Shaking Water Bath - Related Videos

Research

JoVE Journal - Engineering

Ice Generation and the Heat and Mass Transfer Phenomena of Introducing Water to a Cold Bath of Brine

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Cited by 1 •

2017

Here, we present a protocol to demonstrate the generation of ice when water is introduced to a cold bath of brine, as a secondary refrigerant, at a range of temperatures well below the freezing point of water. It can be used as an alternative way of producing ice for industry.

Vacuum-Sealed Hot Water Bath Immersion for the Preparation of Anatomical and Surgical Cadaveric Bone Models

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Cited by 1 •

2022

The present protocol describes the maceration and cleaning of cadaveric bone with a vacuum-sealed, hot water bath immersion technique. This is a low-cost, safe, and effective method to produce anatomical specimens for surgical planning and medical education as an alternative to three-dimensional (3D) printed models.

Research

JoVE Journal - Biology
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Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research

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Cited by 75 •

2015

This protocol describes the measurement of isometric contraction in an isolated smooth muscle preparation, using an isolated tissue bath system and computer-based data acquisition.

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

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Cited by 12 •

2016

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

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Cited by 6 •

2019

Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.

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