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An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

DOI:
10.3791/57922-v

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05:40 min

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January 07, 2019

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, , ,
1Department of Chemical System Engineering,University of Tokyo, 2Department of Biotechnology,Osaka University, 3Biotech Business Unit,Fukoku Co. Ltd, 4Institute of Industrial Science,University of Tokyo

Chapters

  • 00:04Title
  • 01:18Seeding Cells in an O-shaped Bag
  • 02:42Medium Change
  • 03:11Cell Collection
  • 04:07Results: Morphology and Viability of Aggregates
  • 05:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.

Tags

Keywords: Orbital Shaking CultureMammalian CellsO-shaped VesselsUniform AggregatesDrug ScreeningSuspension CultureHEK-293 CellsCell AggregationCell CultureBioreactor Design

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