Toxoplasma Growth
Toxoplasma Growth - Related Videos
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
09:49
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
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Cited by 13 •
2020
Presented here is a protocol to evaluate the inhibition efficacy of chemical compounds against in vitro intracellular growth of Toxoplasma gondii using a luciferase-based growth assay. The technique is used to confirm inhibition specificity by genetic deletion of the corresponding target gene. The inhibition of LHVS against TgCPL protease is evaluated as an example.
14:56
A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
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Cited by 18 •
2012
Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.
09:56
Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
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Cited by 27 •
2010
Toxoplasma gondii converts to a cyst form in response to environmental stresses, which can be mimicked in tissue culture models. This video demonstrates techniques to examine cyst wall formation by activating bone marrow-derived macrophages or changing growth medium pH in fibroblast cells.
09:52
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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Cited by 20 •
2013
Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.
FAQs
Endodyogeny allows tachyzoites to produce daughter parasites within the parasitophorous vacuole, the intracellular compartment formed after invasion. This coordinated replication increases parasite numbers while preserving their association with the host cell. Subsequent release supplies tachyzoites capable of entering neighboring cells, making intracellular replication and cell-to-cell spread central outcomes of the culture system.
The parasitophorous vacuole is the site where invading tachyzoites remain associated with the host cell and reproduce. Its formation links invasion to intracellular multiplication, so researchers can examine how parasite entry, replication, and release occur as connected stages. Monitoring this compartment therefore helps interpret changes in parasite propagation during biological experiments.
Host-cell type is a major controlled variable because Toxoplasma gondii must occupy susceptible cells to continue its growth cycle. The selected cells provide the setting for invasion, vacuole formation, tachyzoite replication, and eventual release. Comparing or controlling host-cell types can therefore affect how consistently parasites are maintained and how host-pathogen interactions are studied.
Nutrients, temperature, and passage timing are regulated to keep the host-cell environment suitable for continued parasite propagation. These conditions influence whether infected cells remain able to support intracellular replication and whether newly released tachyzoites can establish further infections. Coordinating them helps maintain cultures and prevents the growth process from becoming poorly controlled over successive passages.
A culture workflow follows the progression from tachyzoite invasion of susceptible host cells to reproduction within parasitophorous vacuoles and release from infected cells. Researchers then use the released parasites to infect neighboring cells or continue the culture through appropriately timed passages. Tracking these stages provides a practical framework for maintaining material and assessing propagation.
Cultured Toxoplasma provides parasite material for investigating cellular invasion, parasite biology, and host-pathogen interactions. The same growth systems support drug screening, genetic manipulation, and evaluation of strategies intended to control toxoplasmosis. Because the culture captures invasion, intracellular replication, and release, it can connect mechanistic observations with experimental testing of interventions.