Bacterial Motility

Bacterial motility is the ability of bacteria to move through liquid environments or across surfaces, shaping how they find nutrients, avoid harmful conditions, and colonize new habitats. Many bacteria swim using flagella, whose rotary motors convert the proton motive force across the cell membrane into movement, while others spread through surface-associated mechanisms such as type IV pilus retraction. Chemotaxis receptors detect chemical gradients and adjust flagellar activity, directing cells toward favorable conditions or away from repellents. Studying bacterial motility helps explain infection, biofilm formation, environmental adaptation, and microbial behavior, while supporting research on antimicrobial strategies and engineered biological systems.

Bacterial Motility - Related Videos

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JoVE EoE - Bacterial Growth and Techniques

Assessing Bacterial Motility Using a Microfluidic Device

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2025

Source: Scheidweiler, D., et al. Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales. J. Vis. Exp. (2020).This video demonstrates the use of a microfluidic device to quantify bacterial motility through a porous matrix composed of randomly arranged pillars. Fluorescently labeled bacteria are introduced into the device, imaged via microscopy as they move through the porous matrix into the observation channel, and analyzed...

Swarming Assay for Visualizing Bacterial Surface Motility

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2025

Source: Morales-Soto, N. et. al., Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays. J. Vis. Exp. (2015)This video demonstrates a swarming assay using bacteria that express green fluorescent protein to observe surface motility on soft agar. Time-lapse imaging captures coordinated expansion and tendril formation, highlighting bacterial surface motility.

Research

JoVE Journal - Biology
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Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

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Cited by 68 •

2015

Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

Visualization of Bacterial Motility Using Redox Dye–Supplemented Semisolid Medium

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2025

Source: Chu, W., and Zhuang, X. Visualizing Bacterial Motility Based on a Color Reaction. J. Vis. Exp. (2022)This video demonstrates a redox dye-based semisolid agar assay for assessing bacterial motility by visually distinguishing motile and non-motile strains.

Research

JoVE Journal - Immunology and Infection

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

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Cited by 2 •

2022

Here, we describe the construction of null mutants of Aeromonas in specific glycosyltransferases or regions containing glycosyltransferases, the motility assays, and flagella purification performed to establish the involvement and function of their encoded enzymes in the biosynthesis of a glycan, as well as the role of this glycan in bacterial pathogenesis.

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