Biotinylated Rrna Probes
Biotinylated Rrna Probes - Related Videos
06:15
Biotinylated Cell-Penetrating Peptide Probe to Detect Protein-Protein Interactions
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2025
This video demonstrates a technique that uses biotinylated cell-penetrating peptides to detect intracellular protein-protein interactions. The cell-penetrating peptides facilitate the conjugated peptide to enter a cell and specifically bind to its interacting proteins. The biotin, after binding to avidin, allows purification of the interacting proteins’ complex from a cell lysate.
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
11:15
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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Cited by 16 •
2015
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
10:57
16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species
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2023
Source: Ewa Bukowska-Faniband1, Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Planet Earth is a habitat for millions of bacterial species, each of which has specific characteristics. Identification of bacterial species is widely used in microbial ecology to determine biodiversity of environmental samples and medical microbiology to diagnose infected patients. Bacteria can be classified...
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
FAQs
Target recognition depends on sequence complementarity: the probe binds a matching region of ribosomal RNA through complementary base pairing. A probe directed at one rRNA sequence can therefore distinguish cells or microbial groups associated with that sequence, provided the target region is present. This sequence-level interaction gives the approach selective detection in mixed biological samples.
The biotin tag connects the hybridized probe to a detectable or recoverable output. Biotin binds strongly to streptavidin or avidin, which can be linked to a fluorescent molecule, an enzyme, or a magnetic particle. This interaction converts an otherwise sequence-level recognition event into a visible signal or a means of nucleic acid capture.
The linked detection component determines how researchers use the probe. Fluorescent molecules support visualization by microscopy, enzymes provide a detectable signal, and magnetic particles enable recovery or capture of material associated with the target. Thus, the same biotin-based recognition strategy can support imaging, detection, or isolation, depending on the attached streptavidin or avidin-linked component.
Ribosomal RNA sequence information provides a basis for associating a probe with particular cells, organisms, or microbial groups. When those targets occur together in a complex sample, probe binding can connect sequence identity with measurable location or abundance. This makes rRNA-directed detection useful for examining which groups are present and how they are distributed.
The essential workflow begins with hybridization, during which the probe contacts the sample and recognizes a complementary rRNA region. The biotin on any bound probe then interacts with a streptavidin- or avidin-linked fluorescent molecule, enzyme, or magnetic particle. The resulting output can be observed, measured, or recovered according to the selected linked component.
In fluorescence in situ hybridization, the probe’s sequence recognition is paired with a fluorescent detection component attached through the biotin and streptavidin or avidin interaction. Microscopy can then reveal where target cells or microbial groups occur within the examined sample. This application connects identity with spatial distribution rather than reporting sequence information without cellular context.
Biotinylated rRNA probes can contribute to microbial identification and help assess abundance, spatial distribution, and community composition. In complex samples, different target sequences allow observations to be associated with particular microbial groups. Their value lies in linking molecular recognition to visible or recoverable outputs, supporting analysis of which groups occur, where they occur, and how communities are organized.