Bronchial Aspirate
Bronchial Aspirate - Related Videos
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
14:39
Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
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Cited by 4 •
2010
Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks.
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
01:23
The Bronchial Tree
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2025
The human bronchi and bronchial tree play a crucial role in the respiratory system, facilitating the exchange of oxygen and carbon dioxide. Let's delve into the intricate structure and functions of these respiratory components. The trachea, commonly known as the windpipe, is a tube that connects the larynx (voice box) to the bronchi. At a point called the carina, it bifurcates into two primary bronchi. The right primary bronchus is wider, shorter, and more vertical than the left primary...
09:12
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
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Cited by 13 •
2016
Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.
FAQs
Negative pressure removes airway secretions from the bronchi through an endotracheal tube or bronchoscope. This mechanical step concentrates mucus and cellular material from the lower respiratory tract, allowing subsequent testing to examine airway-associated organisms, inflammatory responses, or abnormal cells. Because collection targets bronchial contents, the sample can connect local airway findings with broader respiratory disease processes.
Each analysis provides a different view of the sample. Microscopy can show cellular or microbial features, culture can support investigation of microorganisms, molecular testing can detect microbial signals, and cytology can examine cells for abnormal characteristics. Using these approaches together broadens interpretation by linking the aspirate’s biological contents with infection, inflammation, or disease-related cellular changes.
Secretions from the upper airways could obscure findings that originate in the bronchi. Collection through an endotracheal tube or bronchoscope, combined with targeted suction, is intended to limit that contamination. A specimen that better represents lower-airway material strengthens the biological interpretation of detected microorganisms, inflammatory responses, or abnormal cells.
Collection requires access to the bronchi through an endotracheal tube or bronchoscope, followed by suction with negative pressure. The resulting material contains mucus and cells available for laboratory examination. This workflow emphasizes targeted sampling rather than general respiratory secretion collection, helping investigators study lower-airway biology while preserving material for multiple analytical approaches.
Researchers can use these samples when they need biological information from the lower respiratory tract. Supported applications include investigating respiratory infections, airway inflammation, lung disease, and responses to treatment. Because the sample contains both secretions and cellular material, it can support parallel examination of microorganisms, host inflammatory activity, and cellular abnormalities within respiratory research.
Findings from bronchial aspirates can be related to disease mechanisms and treatment response. Microorganisms may inform infection investigations, inflammatory findings may indicate airway biological activity, and cytological abnormalities may contribute to lung-disease research. In clinical research, comparing these sample results with patient outcomes helps examine how lower-airway processes correspond to illness and therapeutic effects.