Carbohydrate Lipid Protein

Carbohydrates, lipids, and proteins are three major classes of biological molecules that provide energy, build cellular structures, and control essential processes in living organisms. Carbohydrates form through glycosidic bonds between sugars, lipids commonly assemble from glycerol and fatty acids through ester bonds, and proteins arise when amino acids join by peptide bonds and fold into specific three-dimensional shapes. Their chemical structures determine properties such as solubility, energy storage, membrane formation, catalysis, and molecular recognition, making these molecules central to metabolism, cell signaling, genetics, nutrition, and biomedical research.

Carbohydrate Lipid Protein - Related Videos

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JoVE Journal - Biology
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Crystallization of Membrane Proteins in Lipidic Mesophases

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Cited by 30 •

2011

The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

Research

JoVE Journal - Biology
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

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Cited by 48 •

2010

Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.

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JoVE Journal - Biochemistry
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Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

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2025

This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.

Research

JoVE EoE - Biomolecular Interaction Detection Techniques

Microscale Thermophoresis to Study Protein-Lipid Interactions in Solution

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2025

This video demonstrates microscale thermophoresis for studying the protein-lipid interaction. The assay detects the interaction between molecules by quantifying the thermophoretic movement of fluorescent-labeled proteins in response to a temperature gradient. The fluorescent molecule is mixed with different concentrations of the non-fluorescent lipid molecules, and the mixture of molecules in the solution is loaded into capillaries. A temperature gradient is applied to the samples in the...

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

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Cited by 27 •

2012

Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well...

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