Aspartate Transcarbamoylase
Aspartate Transcarbamoylase - Related Videos
07:48
Preparing a 68Ga-labeled Arginine Glycine Aspartate (RGD)-peptide for Angiogenesis
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Cited by 3 •
2019
The αvβ3 integrin is a type of adhesion protein that is highly expressed on activated endothelial cells undergoing angiogenesis. Thus, evaluating the integrity of the integrin is of great interest in oncology. Here, we introduce a method to prepare 68Ga-labeled radiopeptides and a method to assess its biological effectiveness.
10:19
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
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Cited by 15 •
2013
Over the recent years, live cell-based assays have been used successfully to detect antibodies against surface and conformational antigens. Here, we describe a method using high-throughput flow cytometry enabling the analysis of large cohorts of patients. Detection of novel antibodies will improve diagnosis and treatment of immune-mediated disorders.
00:49
Ligand Binding and Linkage
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2020
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
07:11
Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity
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2025
Here, we describe the use of a genetically directed alkaline phosphatase (AP) labeling method to investigate the anatomical features and phenotypes of mouse retinas under NMDA-induced excitotoxicity. In conjunction with several retinal ganglion cell (RGC) subtype-specific reporter lines, we uncover novel phenotypes in dying RGCs throughout the degenerative process.
07:20
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
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Cited by 1 •
2018
We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
FAQs
CTP acts as a feedback signal from the pyrimidine nucleotide pathway, reducing aspartate transcarbamoylase activity when pyrimidine production is already sufficient. This links enzyme output to the pathway’s end products rather than allowing uninterrupted synthesis. The mechanism illustrates how metabolic pathways can limit unnecessary investment in producing DNA and RNA building blocks.
ATP can promote activity in many bacterial systems, providing a regulatory influence that contrasts with CTP inhibition. This opposing effect helps relate pyrimidine production to the broader nucleotide environment of the cell. Studying the balance between these signals shows how allosteric enzymes integrate information about metabolic demand instead of responding only to substrate availability.
The association of catalytic and regulatory subunits allows structural communication within aspartate transcarbamoylase. Binding of regulatory molecules can therefore influence catalytic behavior through changes transmitted across the enzyme complex. This organization makes the protein useful for examining cooperativity, in which activity changes reflect interactions among parts of a multisubunit enzyme rather than isolated active-site chemistry.
Its position in pyrimidine biosynthesis, sensitivity to feedback inhibition, and response to ATP connect molecular structure with pathway-level regulation. Researchers can use these relationships to examine how an enzyme controls metabolic flow and how regulatory signals modify catalysis. The enzyme consequently provides a focused biological system for linking protein behavior with cellular nucleotide production.
A conceptual investigation compares enzyme activity under regulatory influences associated with CTP and ATP, while considering the roles of the catalytic and regulatory subunits. The resulting activity patterns can reveal whether signals inhibit or promote pathway entry. Such comparisons help connect allosteric behavior with control of pyrimidine nucleotide biosynthesis in bacterial systems.
Aspartate transcarbamoylase regulates an early step that contributes to forming pyrimidine nucleotides, the building blocks required for DNA and RNA. Changes in its activity can therefore influence how strongly the pathway produces these compounds. Studying its regulation helps explain how cells coordinate nucleotide biosynthesis with the need for genetic material and related cellular processes.