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JoVE Journal
Biology

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Crystallization of Membrane Proteins in Lipidic Mesophases
 

Crystallization of Membrane Proteins in Lipidic Mesophases

Article DOI: 10.3791/2501 11:53 min
March 28th, 2011

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Summary March 28th, 2011

The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

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