31,220 Views
•
11:53 min
•
March 28, 2011
DOI:
Summary
Automatically generated
The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.
Explore More Videos
Related Videos
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Related Videos
29916 Views
09:23
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
Related Videos
11159 Views
07:26
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
Related Videos
23572 Views
20:21
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Related Videos
17483 Views
18:45
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
Related Videos
24776 Views
08:23
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Related Videos
23584 Views
11:53
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Related Videos
15436 Views
08:46
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Related Videos
32597 Views
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
Related Videos
9651 Views
07:08
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
Related Videos
13679 Views
Read Article
Cite this Article
Liu, W., Cherezov, V. Crystallization of Membrane Proteins in Lipidic Mesophases. J. Vis. Exp. (49), e2501, doi:10.3791/2501 (2011).
Copy