Citrus Self-incompatibility
Citrus Self-incompatibility - Related Videos
09:23
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
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Cited by 5 •
2018
Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.
11:30
Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
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2023
We engineered, fabricated, and validated an instrument that rapidly processes phloem-rich bark citrus budwood tissues. Compared to current methods, the budwood tissue extractor (BTE) has increased sample throughput and decreased the required labor and equipment costs.
07:12
Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
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2023
This protocol provides a rapid method for determining pollen compatibility and incompatibility in citrus cultivars.
11:39
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
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Cited by 39 •
2012
Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.
06:13
Vertical T-maze Choice Assay for Arthropod Response to Odorants
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Cited by 18 •
2013
A vertical, T-maze olfactometer is described for assaying the behavioral response of arthropods. The olfactometer allows the experimenter to measure choices performed by test subjects when subjected to two potential odor fields. Both attraction to and repulsion from odorants can be measured with this device.
FAQs
Recognition takes place within the plant’s genetic compatibility system as pollen interacts with reproductive tissues. When the pollen is incompatible, the pollen tube is arrested before it reaches the ovule, preventing the reproductive sequence from progressing to fertilization. This checkpoint explains why pollen may be present on a stigma without producing seeds.
The style provides the pathway through which a pollen tube must grow toward the ovule. In an incompatible combination, pollen-tube growth is stopped within this route before the ovule can be reached. The location of this arrest helps connect genetic recognition with practical outcomes such as reduced seed formation and unsuccessful self-pollination.
Compatible pollen can germinate on the stigma and continue tube growth toward the ovule, allowing fertilization to occur. Incompatible pollen is recognized and stopped before that point. Consequently, the same plant may show different fruit-set or seed-formation results depending on which pollen source is involved, making pollen relationships important in citrus reproduction.
Orchard planning should consider whether neighboring cultivars have compatible pollen relationships. When self-pollination or a particular pairing is ineffective, growers may need compatible pollinizers to provide usable pollen. This arrangement can improve the likelihood of successful fertilization and support more predictable fruit set and seed formation across the planting.
A breeding plan begins by identifying the pollen relationships among the selected cultivars. Breeders then choose compatible parents and arrange managed cross-pollination so pollen reaches the intended stigma. Afterward, fruit set and seed formation provide practical outcomes for evaluating whether the selected cross supported successful reproduction and should be used further.
The trait influences how citrus plants exchange genetic material and therefore affects breeding design and conservation of citrus diversity. Researchers must account for compatibility when selecting crosses intended to produce seed or combine parental characteristics. Understanding these relationships can help develop cultivars with more predictable reproductive performance rather than relying on ineffective pollen pairings.