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JoVE Journal
Biology

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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
 

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Article doi: 10.3791/3928
September 21st, 2012

Summary September 21st, 2012

Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

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