Cre-inducible Reporter
Cre-inducible Reporter - Related Videos
02:45
Assessment of Viral Fusion Inhibitors Using a Cre-Inducible Fluorescent Reporter
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2026
Source: Esposito, A. M.,et al. High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (2018)This video demonstrates a co-culture fluorescent assay to monitor cell-to-cell viral transmission. The assay uses a red-to-green reporter cassette to visualize viral membrane fusion and evaluate the efficacy of test compounds in blocking viral entry.
08:19
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
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Cited by 15 •
2011
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
03:12
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
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2025
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
01:59
Cre-LoxP Mediated Induction of Cerebral Cavernous Malformations in a Mouse Model
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2025
In this video, a genetically modified mouse pup carrying the Ccm2 gene flanked by loxP sites and Cre recombinase fused to an estrogen receptor is used to induce cerebral cavernous malformations (CCMs). The administration of 4-hydroxytamoxifen activates Cre in endothelial cells, leading to the deletion of Ccm2 and the formation of CCMs in the brain.
10:29
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Cited by 6 •
2017
Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.
FAQs
Activation depends on site-specific recombination at loxP sites. Cre recombinase recognizes the two loxP-flanked boundaries and excises the intervening transcriptional stop cassette. Once that block is removed, the downstream reporter can be expressed under control of the relevant promoter or enhancer. This molecular switch converts Cre activity into a detectable marker, linking recombination to cell identification.
Cre specifies the cells in which the stop cassette is removed, whereas the promoter or enhancer determines how reporter expression is driven after activation. This separation lets a design connect a genetically defined cell population with a chosen readout, such as fluorescence or another marker. The resulting pattern can therefore reflect both Cre activity and regulatory control of the reporter.
Lineage tracing relies on the inherited record created when Cre activates the reporter in a cell. The initially labeled population can then be followed through its descendants, allowing researchers to examine long-term changes and developmental patterns rather than only the cells present at the moment of observation. This connects an early cell state with later tissue organization.
A Cre-inducible reporter can provide either a snapshot of cell-specific gene activity or a longer-term record of a genetically defined population, depending on the biological question and reporter design. Mapping current signal helps identify where activation occurs, while following labeled descendants addresses lineage relationships. Distinguishing these uses prevents tissue location and developmental history from being treated as the same measurement.
Using this approach begins with a reporter construct in which a loxP-flanked transcriptional stop sequence lies before the marker gene. Researchers then pair that construct with Cre activity associated with the cell population of interest and examine the resulting reporter signal. The workflow links construct design, cell-specific recombination, and detection of fluorescence or another marker.
Fluorescent proteins provide a visible readout, while other markers can supply alternative detectable signals. The choice of reporter determines what can be observed when the stop cassette has been excised, but the central interpretation remains the same: signal marks cells in which Cre enabled reporter expression. These outputs support identification of defined populations and visualization of their distribution.
In living organisms, researchers apply these reporters to map tissues, trace cell lineages, and analyze cell-specific gene activity. The approach can reveal developmental patterns, cellular behaviors, and long-term changes in genetically defined populations. Because the signal is tied to Cre-dependent activation, observations can be related back to a selected cellular population rather than interpreted as an undifferentiated tissue-wide measurement.