Liposome Preparation
Liposome Preparation - Related Videos
09:03
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
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Cited by 23 •
2013
Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.
10:43
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
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Cited by 17 •
2018
Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.
10:33
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
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Cited by 18 •
2016
The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.
03:54
Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
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2025
This video showcases the assessment of B cell activation through the utilization of antigenic liposomes. B cells treated with calcium-sensitive Indo-1 dye are distinguished using flow cytometry, and their violet-to-blue fluorescence ratio is analyzed. As the cells encounter antigenic liposomes, it triggers B cell activation through calcium influx, progressively enhancing the violet-to-blue fluorescence signal ratio over time.
02:57
Targeted siRNA Delivery to Neurons for Prion Protein Silencing Using Liposomal Complexes
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2025
Source: Bender, H. R., et.al. Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes. J. Vis. Exp. (2016)This video demonstrates the targeted delivery of prion protein (PrP) siRNA to neurons using liposomal siRNA-peptide complexes (LSPCs). Cationic liposomes electrostatically bind PrP-targeting siRNA, which is further stabilized by the RVG-9r peptide for receptor-mediated transport across the blood-brain barrier. Once inside the brain, LSPCs undergo endocytosis in...
FAQs
Hydration supplies the aqueous environment that allows the thin lipid film to reorganize into vesicles. The phospholipids form bilayers while enclosing part of the surrounding water, producing the aqueous core. This step therefore establishes the vesicle architecture that later processing can modify to achieve more consistent size and experimental performance.
Composition, size, and surface properties are central determinants of liposome behavior. They can influence stability, cellular uptake, biodistribution, and overall experimental performance. Adjusting these features allows investigators to study membrane behavior or develop carriers whose interactions with biological systems differ according to the intended application.
Encapsulation efficiency indicates how effectively a preparation incorporates a drug, nucleic acid, protein, or imaging agent into the vesicles. It directly affects how much material the preparation can carry and helps researchers evaluate experimental performance. Comparing efficiency across formulations can reveal whether changes in composition or processing improve the carrier's usefulness.
Both extrusion and sonication can reduce and standardize liposome size after hydration, but they represent different processing options. Their role is to modify the size distribution established during vesicle formation rather than create the initial lipid bilayer architecture. Size control matters because vesicle dimensions can influence stability, uptake, biodistribution, and experimental consistency.
A typical workflow dissolves the selected lipids in an organic solvent, removes that solvent to produce a thin lipid film, and hydrates the film to generate vesicles. Extrusion or sonication may follow when smaller or more standardized vesicles are needed. The resulting formulation is then assessed through properties relevant to its biological use.
Researchers use liposomes as simplified models of cellular membranes and as carriers for drugs, nucleic acids, proteins, or imaging agents. The appropriate role depends on the experimental objective: a model can support membrane-focused studies, whereas a carrier formulation can examine delivery or imaging performance in biological systems.
Evaluation should consider stability, cellular uptake, biodistribution, and experimental performance, along with size, surface properties, and encapsulation efficiency. These measurements connect formulation characteristics with biological behavior. For example, a preparation may be technically successful yet perform poorly if its properties do not support adequate stability, uptake, distribution, or cargo incorporation.