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Preparation, Purification, and Use of Fatty Acid-containing Liposomes
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

DOI:
10.3791/57324-v

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10:43 min

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February 09, 2018

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, , ,
1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology,Massachusetts General Hospital, 2Department of Biomedical Engineering,Boston University, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Chapters

  • 00:05Title
  • 00:50Vesicle Preparation
  • 04:43Vesicle Purification
  • 06:59Use of Vesicles in the Presence of Magnesium
  • 07:53Hammerhead RNA Self Cleavage in Vesicles
  • 09:02Results: Hammerhead Ribozyme Cleavage in Oleic Acid/Glycerol Monooleate Vesicles
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.

Tags

Keywords: Fatty AcidLiposomesVesiclesSynthetic BiologyOrigin Of LifeMinimal CellsArtificial ProtocellsThin FilmRehydrationExtrusionPolycarbonate MembraneTris-HCL Buffer

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