Mammalian Cell Infection
Mammalian Cell Infection - Related Videos
03:02
Oncolytic Virus Purification from Infected Mammalian Cells
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2026
Source: Nguyen, H., et al. Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus. J. Vis. Exp. (2021)This video demonstrates the purification of an oncolytic virus from infected mammalian cell cultures using nuclease treatment, sequential centrifugation, membrane filtration, and sucrose cushion centrifugation.
13:54
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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Cited by 2 •
2013
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
02:52
Expansion of Mutant Vaccinia Virus in Mammalian Cells
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2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the expansion of plaque-derived recombinant virus in adherent mammalian cells. The virus carries a fluorescent reporter in place of a virulent gene, enabling infection tracking. Infected cells are harvested for PCR verification of the genetic modification.
05:57
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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Cited by 81 •
2011
This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
09:20
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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Cited by 29 •
2010
In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.
FAQs
Specific host-cell receptors help determine whether a pathogen can attach to a cultured mammalian cell and begin entry or internalization. This receptor-dependent step connects pathogen surface features with cellular susceptibility, making it important for analyzing infection mechanisms. Comparing cellular responses after attachment and entry can help researchers distinguish effects linked to recognition from those associated with later intracellular events.
After entry, a pathogen may replicate within the cell or persist without producing the same pattern of activity. This distinction affects how researchers interpret infection progression, cellular damage, and host responses. Studying both outcomes helps connect intracellular pathogen behavior with longer-term host-pathogen interactions and supports evaluation of strategies intended to control infection.
Infected mammalian cells can activate inflammatory and antiviral responses as they detect or respond to the pathogen. These defenses may influence pathogen replication, persistence, and the condition of the host cell, while the pathogen may alter the interaction in return. Measuring these responses helps researchers examine immune signaling alongside direct effects such as cytotoxicity.
A study generally follows the sequence of exposing cultured mammalian cells to a pathogen, examining attachment and entry or internalization, and then assessing replication or persistence. Researchers also evaluate cellular defenses, immune signaling, and possible cytotoxic effects. Organizing observations across these stages helps separate early interaction events from later biological consequences.
Infected cultures can provide information about host-pathogen interactions, infection mechanisms, cytotoxicity, and inflammatory or antiviral signaling. Researchers can relate pathogen behavior to changes in the host cell and determine whether infection produces evidence of replication, persistence, or cellular injury. These outcomes make the model useful for connecting molecular events with broader biological and medical questions.
These models are used to evaluate vaccines, antiviral or antimicrobial compounds, and other therapeutic strategies in the context of infected cells. Researchers can examine whether an intervention changes pathogen behavior, cellular damage, or host defensive signaling. Because the system links infection events with measurable cellular outcomes, it provides a controlled setting for comparing potential approaches before broader biological or medical investigation.