Ipsc-derived Neurons
Ipsc-derived Neurons - Related Videos
05:06
Three-Dimensional Thymic Culture System to Generate iPSC-Derived Thymic Emigrants
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2025
This video demonstrates a protocol for generating functional antigen-specific T cells from iPSC-derived immature T cells using a three-dimensional thymic culture system. Co-culturing the iPSC-derived immature T cells with endogenous lymphocyte-depleted mice fetal thymic lobes resulted in successful maturation of the T cells to form CD8αβ+ MHC class I+ iPSC-derived thymic emigrants (iTEs).
07:40
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
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Cited by 46 •
2017
Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.
05:23
Developing Human iPSC-Derived Motor Nerve Organoids Using a Microfluidic Chip
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2025
This video demonstrates the method of creating motor nerve organoids by first culturing human induced pluripotent stem cells in a special medium to form spheroids, then differentiating them into motor neurons using specific inhibitors, and finally cultivating them in a microfluidic chip to extend axons and form motor nerve organoids.
07:53
Generating a Blood-Brain Barrier Chip Using iPSC-derived Precursor Cells
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2025
This video showcases creating a blood-brain barrier chip using iPSC-derived precursor cells. It involves seeding neural progenitor cells and brain microvascular endothelial cells in separate channels with a porous membrane. By flowing differentiation media, the endothelial cells form tight junctions resembling blood vessels, and the neural cells mature into various brain cell types, establishing a functional blood-brain barrier on the chip.
08:03
Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications
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Cited by 4 •
2023
Here, we present a protocol to produce 3D-bioprinted cocultures of iPSC-derived neurons and astrocytes. This coculture model, generated within a hydrogel scaffold in 96- or 384-well formats, demonstrates high post-print viability and neurite outgrowth within 7 days and shows the expression of maturity markers for both cell types.
FAQs
Timed developmental signals guide cells through a sequence that resembles neural development rather than prompting neuronal formation all at once. Their timing helps move induced pluripotent stem cells toward neural progenitor stages and then supports progression toward specialized neuronal subtypes. This staged approach is important because neuronal identity, morphology, and function emerge as cells experience the appropriate developmental conditions.
Neural progenitor stages provide an intermediate point between the stem-like state of iPSCs and mature neurons. Passing through these stages allows researchers to direct development toward neuronal cells instead of relying on an undifferentiated population. The staged progression also creates a framework for studying developmental changes and for generating cells with more defined neuronal characteristics.
Specialization develops when cells receive developmental signals and culture conditions that promote particular neuronal identities and maturation. As this process continues, cells develop characteristic morphology and functions associated with their subtype. These features give researchers observable and functional properties for examining how neuronal cells develop and for comparing disease-related changes in controlled experimental systems.
Defined culture conditions influence whether cells progress efficiently from iPSCs through neural progenitor stages and toward mature neuronal states. They work together with the timing of developmental signals to support neuronal specialization and maturation. Consistent conditions are therefore important when researchers compare cell populations, investigate cellular mechanisms, or evaluate changes associated with neurological disease.
The workflow begins with induced pluripotent stem cells and applies timed developmental signals under defined culture conditions. Cells are guided first toward neural progenitor stages, then encouraged to mature into specialized neuronal subtypes. Researchers can subsequently examine characteristic morphology and functions, using the resulting cells as controlled models for development, disease mechanisms, neurotoxicology, or treatment evaluation.
They are useful when researchers need human-relevant experimental models for brain development, neurological disease, or neurotoxicology. Because the cells can be studied under controlled conditions, investigators can examine neuronal behavior and cellular mechanisms in a laboratory system. The same platform also supports drug screening and assessment of how candidate treatments affect neuronal cells.
Studies can reveal disease-related neuronal changes by comparing cellular characteristics and functions in relevant experimental systems. They can also support evaluation of potential treatments and screening of drugs under controlled conditions. These outcomes connect cellular observations with broader questions about neurological disease, allowing researchers to investigate mechanisms and assess whether interventions produce useful effects in neuronal models.