Miltenyi Biotech System
Miltenyi Biotech System - Related Videos
09:47
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
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Cited by 19 •
2007
Isolation of lymphocytes using the Miltenyi MACs kit is a reliable way to purify cells from whole lymphoid tissue homogenates. Cells purified using the Miltenyi system are typically ≥ 96% pure. Here, we demonstrate the steps taken to isolate CD4+ T cells, one of the many kits offered by Miltenyi.
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Cited by 22 •
2008
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
07:03
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
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Cited by 45 •
2009
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
11:17
Generation of Single-Cell Suspensions from Mouse Neural Tissue
0 Views •
Cited by 16 •
2009
Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.
12:38
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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2008
This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.
FAQs
Separation depends on selective binding between an affinity reagent and a target cell. When the reagent is attached to a magnetic microbead, the labeled cell can interact with the magnetic column, whereas cells without the label are not retained in the same way. This binding step determines which population becomes available for enrichment or removal.
The magnetic field retains cells carrying the attached microbeads inside the column, while unlabeled cells pass through. This creates physically different fractions that can support either target-cell enrichment or depletion strategies. The distinction is important because the resulting fraction, rather than the original mixed sample, is typically used for later biological analysis.
Defined preparations reduce variation caused by differences in the cellular composition of starting samples. A more consistent population can improve the comparability of culture, flow cytometry, molecular analysis, and functional studies. In immunology, cell biology, and stem cell research, this control helps investigators relate observed results more directly to the population under investigation.
A typical workflow begins with a biological sample and an affinity reagent that recognizes the desired cell population. Magnetic microbeads connect the bound reagent to the cells, and the sample is then applied to a magnetic column. The column separates retained labeled cells from cells that pass through, producing fractions for subsequent experiments.
The separated fractions provide cell populations suited to different downstream purposes. Researchers may use them for cell culture, flow cytometry, molecular analysis, or functional studies, depending on the biological question. Because the system can enrich or remove defined populations from complex samples, it supports experiments that require a more controlled cellular starting material.
The platform is useful when investigators need specific cell populations from complex biological samples. Its applications include immunology, cell biology, stem cell research, and clinical research. In these settings, magnetic separation can prepare cells before characterization, culture, molecular testing, or functional assessment, helping align the sample composition with the requirements of the experiment.