Murine Embryonic Fibroblasts
Murine Embryonic Fibroblasts - Related Videos
08:57
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Cited by 3 •
2016
Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.
05:54
Rapid Culturing of Primary Murine Melanocytes and Fibroblasts: Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample
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2023
This video describes the rapid method to isolate and culture the melanocytes and fibroblast simultaneously from the postnatal mouse pup’s skin. These cells can be maintained and manipulated in vitro to study skin cell biology, wound healing, and melanoma.
07:12
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
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Cited by 3 •
2012
Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.
09:38
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
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Cited by 170 •
2012
The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
FAQs
Adhesion to a culture surface and subsequent proliferation make MEFs experimentally tractable. Once embryonic tissue has been dissociated, the fibroblasts attach under defined laboratory conditions and expand, creating a population that can be observed or manipulated over time. This progression allows researchers to connect altered gene expression or growth behavior with specific experimental conditions.
Genetic or chemical perturbations change cellular conditions in a controlled experimental system, allowing researchers to examine resulting effects on gene expression and cell behavior. Because MEFs can be expanded in vitro, investigators can compare manipulated and unmanipulated cultures while linking molecular changes to broader cellular outcomes. This supports mechanistic studies rather than observation alone.
Genetically modified MEFs support studies of signaling, development, senescence, and cellular reprogramming. Their value comes from combining a manipulable genetic background with measurable changes in growth, gene expression, or cell behavior. Researchers can therefore use these cells to examine how particular molecular alterations influence processes that connect intracellular mechanisms with broader biological states.
Preparation begins with embryonic tissue isolation and dissociation, followed by culture of the resulting cells under defined laboratory conditions. Fibroblasts adhere to the culture surface and proliferate, enabling expansion before experimental manipulation. This workflow produces a cell population suitable for subsequent genetic or chemical perturbation and for monitoring changes in growth or gene expression.
MEFs can function as feeder layers that support the maintenance of embryonic stem cells. In this application, the fibroblast culture provides the cellular context needed to help sustain stem-cell cultures under laboratory conditions. Their use extends MEF research beyond fibroblast biology, making them relevant to experimental systems focused on preserving embryonic stem-cell properties.
MEFs are especially useful when researchers need an accessible, expandable, and experimentally responsive cell system. They can be genetically or chemically manipulated, then assessed for changes in gene expression, proliferation, or cell behavior. This makes them suitable for standardized experiments that test signaling, development, senescence, reprogramming, or relationships between molecular mechanisms and cellular outcomes.