Pancreatic Duct Cannulation
Pancreatic Duct Cannulation - Related Videos
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
04:12
Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion
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2025
This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.
05:11
Treatment of Middle-segment Pancreatic Benign Tumor Using Laparoscopic Central Pancreatectomy with End-to-end Pancreatic Duct Reconstruction
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2026
This article presents laparoscopic central pancreatectomy with pancreatic duct end-to-end anastomosis as an important surgical approach for middle-segment pancreatic benign and low-grade malignant tumors.
13:17
The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
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Cited by 48 •
2015
Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.
FAQs
Direct duct access links a measurable fluid response to pancreatic activity. By collecting secretions or monitoring ductal flow under controlled conditions, researchers can evaluate exocrine function and digestive enzyme release. The same setup also permits comparison of responses before and after hormonal or pharmacological stimulation, helping connect an intervention with a specific functional change.
Securing the cannula at the duct opening keeps access stable while fluid is collected or flow is monitored. Stability matters because the same access point can be used to observe pancreatic output and introduce an experimental substance. This controlled arrangement helps relate a treatment or stimulus to a measured change in ductal function.
The technique supports analysis of several related pancreatic processes, including exocrine secretion, digestive enzyme release, ductal physiology, and responses to hormonal or pharmacological stimuli. Examining these processes through direct duct access gives biological studies a way to connect pancreatic output with normal functional regulation or experimentally induced changes.
A typical workflow begins by positioning a small catheter at the pancreatic duct opening and securing it in place. Researchers then select whether to collect pancreatic secretions, monitor ductal flow, or introduce a substance. The resulting fluid behavior or functional response is observed under the controlled conditions of the experiment.
Researchers can introduce an experimental treatment or apply hormonal or pharmacological stimulation while maintaining direct access to the duct. They then assess pancreatic secretions or ductal flow as the experimental response. This design allows biological studies to examine how pancreatic exocrine activity and ductal function respond to a defined stimulus.
Direct access to the duct provides a consistent way to examine pancreatic function in both normal and disease-related settings. Researchers can compare secretion, digestive enzyme release, or ductal responses under controlled conditions. These comparisons help identify changes in pancreatic biology associated with disease rather than relying only on indirect observations.