Reporter Gene Delivery
Reporter Gene Delivery - Related Videos
02:11
Reporter Genes
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2021
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. Commonly used reporter...
01:28
Generation of Reporter Gene-Carrying Hepatitis B Pseudoviruses
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2026
Take a mixture of plasmids encoding hepatitis B surface antigens and lentiviral proteins, along with a plasmid containing a fluorescent reporter gene and a packaging signal.Add a transfection reagent, mix thoroughly, and incubate.The positively charged transfection reagent forms complexes with the negatively charged plasmid DNA.Introduce these complexes to a monolayer of human embryonic kidney cells and incubate.The complexes enter the cells via endocytosis.Replace the medium to remove residual...
04:25
Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene
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2025
This video demonstrates an in vivo assay in bacteria to study the interaction of RNA-binding proteins (RBPs) with RNA. The bacterial cells are transformed with two plasmid constructs — a binding-site plasmid encoding an mRNA containing a fluorescent reporter gene downstream of an RBP-binding site — while an RBP plasmid expresses the RBP proteins under the control of an inducer. Upon inducer-mediated increase in RBP production, the RBPs bind to the mRNA, resulting in translational repression of...
05:12
Subpial Gene Delivery in the Mouse Spinal Cord to Evaluate Gene Expression
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2025
Source: Tadokoro, T., et al. Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice. J. Vis. Exp. (2017)This video demonstrates a subpial injection technique for gene delivery to the spinal cord in mice. The procedure involves exposing the dura mater, puncturing the pia mater, and injecting a recombinant adeno-associated virus (rAAV) carrying a fluorescent protein-expressing gene into the subpial space. Following injection and recovery, the virus facilitates gene expression in...
07:48
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
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Cited by 10 •
2013
Implantation of cancer cells into the organ of origin can serve as a useful preclinical model to evaluate novel therapies. MB49 bladder carcinoma cells can be grown within the bladder following intravesical instillation. This protocol demonstrates catheterization of the mouse bladder for the purpose of tumor implantation and adenoviral delivery.
FAQs
The promoter determines which regulatory activity the reporter reflects. Placing the reporter gene under a selected promoter allows its signal to indicate whether that regulatory sequence is active in the examined cells or organism. Consequently, promoter choice connects the measured fluorescence, luminescence, or color change to a particular gene-regulation question rather than to cellular activity in general.
Plasmid transfection and viral transduction provide two routes for placing a reporter construct into cells. The overview identifies both as commonly used delivery approaches, but they represent distinct experimental strategies for introducing the gene. Choosing between them can influence how researchers evaluate delivery efficiency and how they apply the reporter system in cultured cells or model organisms.
These signals are measurable outputs produced by the reporter protein, but their meaning depends on the regulatory sequence controlling the construct. A stronger or weaker signal is interpreted as evidence of corresponding promoter activity or pathway behavior within the experimental system. The selected detection mode therefore determines how gene regulation or cellular responses become observable.
A typical workflow begins by selecting a reporter gene and placing it under a promoter relevant to the biological question. Researchers then introduce the construct through plasmid transfection or viral transduction, allow reporter expression to occur, and measure the resulting fluorescence, luminescence, or enzymatic color change. The signal is subsequently related to promoter activity, pathway behavior, or delivery efficiency.
This approach is useful when investigators need a measurable readout of regulatory activity inside cells. A reporter controlled by a selected promoter can reveal changes in gene expression or signaling pathway behavior, while the detectable signal supports comparisons across experimental conditions. It can also help examine cellular localization when the reporter’s distribution is observed within the biological system.
Researchers can use reporter systems to examine whether a genetic construct reaches cells and produces a detectable output, making delivery efficiency an experimental outcome. The same strategy can help evaluate experimental therapies by monitoring gene regulation or biological responses in cultured cells or model organisms. These measurements provide functional context beyond simply confirming that a construct was introduced.