Rnai Gene Silencing
Rnai Gene Silencing - Related Videos
08:47
Gene Silencing with Morpholinos
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2023
Morpholino-mediated gene silencing is a common technique used to study roles of specific genes during development. Morpholinos inhibit gene expression by hybridizing to complementary mRNAs. Due to their unique chemistry, morpholinos are easy to produce and store, which makes them remarkably cost effective compared to other gene silencing methods. This video reviews proper experimental design when using these oligonucleotides. Following that, an explanation of morpholino microinjection...
09:51
RNAi in C. elegans
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2023
RNA interference (RNAi) is a widely used technique in which double stranded RNA is exogenously introduced into an organism, causing knockdown of a target gene. In the nematode, C. elegans, RNAi is particularly easy and effective because it can be delivered simply by feeding the worms bacteria that express double stranded RNA (dsRNA) that is complementary to a gene of interest. First, this video will introduce the concept of RNA interference and explain how it causes targeted gene knockdown.
07:56
Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
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Cited by 18 •
2010
For development of RNA interference (RNAi)-based therapies, a novel strategy was developed, transkingdom RNAi (tkRNAi). This technology uses non-pathogenic bacteria to produce and deliver therapeutic short hairpin RNA (shRNA) into target cells. Here, tkRNAi was successfully applied for reversal of classical ABCB1-mediated multidrug resistance (MDR) of cancer cells.
10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
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Cited by 159 •
2011
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
FAQs
Dicer acts as the processing step that converts double-stranded RNA into smaller RNA molecules, including small interfering RNAs and microRNAs. In this form, the molecules can enter the downstream silencing machinery rather than remaining as the original long duplex. This processing connects the initial RNA signal with sequence-directed control of messenger RNA expression.
After loading into RISC, the guide strand provides the sequence information for recognizing complementary messenger RNA. When this pairing directs cleavage, the target transcript is cut; in other cases, complementary recognition is associated with translational repression, which reduces protein production without producing the same cleavage outcome. Guide-strand complementarity therefore links sequence choice to the type of expression control.
Sequence complementarity gives RNAi gene silencing its selective character. The guide strand is matched to a complementary messenger RNA, allowing the silencing complex to focus on a chosen transcript. That selectivity makes the process useful for linking a gene’s reduced expression with changes in cellular function during biological investigation.
A typical conceptual workflow begins with double-stranded RNA, proceeds through Dicer processing, and then follows loading of a guide strand into RISC. Researchers then examine the consequence of cleavage or translational repression of the complementary messenger RNA. Comparing resulting cellular changes with the targeted gene provides evidence about its function.
Researchers interpret RNAi results by examining how cellular function changes after expression of a selected gene is reduced. If the change aligns with the biological process under study, the experiment can help reveal that gene’s role. This approach is especially useful for modeling disease pathways, where altered cellular behavior can inform investigation of potential therapeutic targets.
RNAi gene silencing supports investigations of development, infection, and cancer, in addition to general studies of cellular function. In each setting, sequence-based targeting allows researchers to reduce expression of a selected gene and observe relevant consequences. It can therefore connect gene activity with biological processes and support evaluation of potential therapeutic targets.