Saline Resuspension
Saline Resuspension - Related Videos
03:15
A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats
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2025
This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.
01:17
Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water
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2025
Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...
06:29
Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
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Cited by 2 •
2016
Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
04:35
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
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2024
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
07:43
Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
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2025
This article describes a protocol for generating arbuscular mycorrhizal (AM) fungi inoculum to investigate AM-enhanced salt stress tolerance in rice.
FAQs
Isotonic saline helps limit osmotic stress when concentrated cells or other biological material are returned to suspension. By reducing the risk of excessive water movement across cell membranes, it supports the preservation of sample integrity and helps minimize cell lysis. This makes the resulting suspension more suitable for measurements and downstream biological analyses.
Uniform dispersion reduces differences in material concentration between portions taken from the same sample. That consistency supports more reliable cell counting, microscopy, washing, dilution, and immunoassay workflows. If the pellet remains unevenly distributed, measurements may reflect sampling differences rather than the true properties of the collected cells, tissue, or particulate material.
The volume of saline and the intensity of mixing both influence the result. An appropriate volume must disperse the concentrated material without creating an unsuitable dilution, while gentle pipetting or mixing should separate the sample without unnecessarily damaging it. These choices affect suspension uniformity, cell lysis, sample loss, and downstream reliability.
Gentle mixing provides enough mechanical action to separate concentrated material while minimizing physical damage and sample loss. This is especially important when the sample contains intact cells whose integrity affects later analysis. Preserving a well-dispersed but minimally damaged suspension improves its usefulness for microscopy, counting, immunoassays, culture, or molecular analysis.
After collection or centrifugation, the concentrated material is retained as a pellet or compact sample. An appropriate volume of isotonic saline is added, and the material is gently pipetted or mixed until it becomes evenly dispersed. The resulting suspension can then be used in the selected downstream workflow, such as counting, washing, dilution, or analysis.
Researchers may use this technique when a collected or centrifuged sample must be prepared for microscopy, cell counting, washing, dilution, or immunoassays. It can also support preparation for downstream culture or molecular analysis. The method is therefore useful whenever concentrated biological material needs consistent handling before observation, measurement, or further processing.
Consistent handling produces suspensions with more comparable material distribution across repeated samples or aliquots. That uniformity supports reliable measurements and helps reduce variation caused by incomplete pellet separation or uneven sampling. In biology workflows, improved consistency is valuable because it strengthens comparisons between observations and supports dependable downstream processing.
A properly prepared suspension can provide material in a form suitable for microscopy, counting, washing, dilution, and immunoassays. It may also serve as an intermediate preparation before culture or molecular analysis. The relevant outcome is not simply dispersion, but a sample that remains sufficiently uniform and intact for the intended biological application.