Subcutaneous Tumor
Subcutaneous Tumor - Related Videos
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
04:13
An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor
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2025
This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.
08:32
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Cited by 9 •
2018
Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
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Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
04:05
Subcutaneous Transplantation Assay: A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model
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2023
This video describes the method for transplantation of tumor cells from the melanoma-bearing donor zebrafish model to an immunocompromised recipient Casper zebrafish. It helps to visualize tumor engraftment and proliferation in the recipient fish.
FAQs
Growth begins when cells acquire changes that disturb controls over proliferation, survival, and tissue organization. These disruptions can allow abnormal cells to persist and expand rather than remain coordinated with neighboring tissue. Studying these altered controls helps biologists connect cellular behavior with the emergence and continued development of a mass beneath the skin.
Continued growth is not determined by abnormal cells alone. The tumor interacts with surrounding connective tissue, blood vessels, and immune cells, making the local environment part of the biological process. Examining these relationships helps researchers study how host tissues respond to tumor development and why the mass may progress within its surroundings.
Changes in local anatomy can alter how nearby tissue is arranged or functions as the mass enlarges. This makes location biologically important, not merely descriptive: investigators can relate the tumor’s position beneath the skin to effects on surrounding structures. Such observations complement cellular analysis when characterizing the lesion.
Their position beneath the skin makes them accessible systems for examining tumor development, progression, and host responses. Researchers can use this setting to connect changes in the tumor with interactions involving connective tissue, blood vessels, and immune cells. The model therefore supports investigation of both tumor behavior and the surrounding biological response.
Clinical evaluation commonly combines physical examination with imaging to assess the mass and its local anatomy. These approaches provide information about the lesion’s observable features and position, while tissue analysis can further establish its cellular characteristics. Together, the methods support a more complete evaluation than any single observation alone.
Tissue analysis helps establish the cellular characteristics of the lesion, adding biological information that physical examination and imaging cannot provide by themselves. This cellular characterization can guide diagnosis in clinical evaluation and support research into tumor development, progression, and interactions with the host tissues surrounding the mass.