Transplantation Model Mice
Transplantation Model Mice - Related Videos
36:30
Small Bowel Transplantation In Mice
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Cited by 2 •
2007
The mouse small bowel transplantation model has been recognized as an important tool to study mechanismes of immune rejection and screen new immunosuppressive drugs. However, this model is limited to use because the techniques involved is an extremely technically challenge. Now we introduce the modified technique.
23:43
Heterotopic Heart Transplantation in Mice
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Cited by 27 •
2007
The mouse heterotopic heart transplantation model has been proven by many investigators to be an important method for studying mechanisms of rejection and immune response. However, the techniques involved are still challenging. By modifying standard techniques we have had success with more than 1000 transplants.
09:10
Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
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Cited by 7 •
2010
This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.
09:48
Heterotopic Cervical Heart Transplantation in Mice
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Cited by 24 •
2015
The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.
10:43
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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Cited by 98 •
2007
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.
FAQs
Immunodeficient strains reduce the likelihood that transplanted material will be rejected by the host. This creates conditions in which human cells or tissues can engraft, proliferate, and interact with mouse physiology. The resulting model helps investigators examine graft behavior in a living organism while limiting immune-mediated loss of the transplanted material.
The mouse microenvironment can influence how transplanted cells or tissues survive, proliferate, and respond to surrounding biological conditions. Consequently, results may reflect both properties of the graft and effects of host physiology. Examining this interaction provides information that isolated cell-based experiments cannot reveal and helps explain variation in graft performance.
Model selection depends on the biological question and the material being transplanted, such as cells, tissues, or organs. Investigators also consider whether reducing graft rejection is necessary and which outcome they need to follow, including graft survival, disease progression, or treatment response. These choices align the host system with the intended experiment.
Cell-based experiments can examine biological behavior under controlled conditions, whereas transplantation model mice add a living host context. This allows investigators to observe transplanted material as it interacts with mouse physiology and to monitor effects over disease progression or treatment. The models therefore provide an intermediate research setting between simplified laboratory systems and clinical studies.
A typical workflow begins by selecting an appropriate mouse strain and transplant material according to the research question. Investigators then introduce the cells, tissue, or organ and monitor the graft within the living host. Subsequent observations focus on survival, proliferation, disease progression, or treatment response, depending on the study objective.
These models support studies of cancer, immune responses, tissue regeneration, and infectious disease. Their value comes from allowing researchers to examine transplanted material in a living organism rather than only in isolated systems. They can also be used to follow disease progression and evaluate how treatments affect graft-associated biological processes.
Researchers can assess whether the transplanted material survives, proliferates, and remains biologically active within the host. They may also follow disease progression and observe responses to treatment. Interpreting these outcomes requires attention to both the graft and the mouse physiology, because host conditions can shape the behavior and effects of the transplanted material.