Cytokine Release Measurement
Cytokine Release Measurement - Related Videos
06:29
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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Cited by 9 •
2011
We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.
02:57
Assessing Antiviral Cytokine Production in Response to Viral Infection
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2026
Source: Zhang, J., et al. Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production. J. Vis. Exp. (2014)This video demonstrates the process of infecting murine fibroblast cells with herpesvirus and monitoring the resulting antiviral cytokine release and gene expression. It outlines key steps, including viral infection, media collection for cytokine quantification, and cell harvesting for gene expression analysis.
12:58
Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
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Cited by 2 •
2017
Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.
06:37
Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety
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2023
The use of cytokine release assay reference reagents allows for more reproducible and standardized in vitro safety profiles of immunotherapeutic monoclonal antibodies. Here we describe how cytokine release assays can be used alongside a reference reagent panel to predict the safety of some therapeutic monoclonal antibodies.
07:30
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
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Cited by 1 •
2012
The amperometric technique measures dopamine release from a single cell by detecting the oxidative current produced by spontaneous dopamine oxidization. Simultaneous voltage clamp and amperometry methodology reveal the mechanistic relationship between the overall "activity" of dopamine transporter and the regulatory role of this activity on the reverse transport of dopamine.
FAQs
Antibody-based formats use target-specific binding to turn secretion into an assay signal. In ELISA, an enzyme-linked detection step generates the readout; multiplex bead-based assays use fluorescence to measure multiple targets, while ELISpot records cytokine-producing activity as spots. These signal formats allow researchers to quantify selected cytokines from culture supernatants or blood and compare cellular responses.
Measuring a panel can reveal a broader response than tracking one cytokine alone. Interferons, interleukins, and tumor necrosis factor provide distinct readouts of immune activation and inflammatory communication, helping investigators characterize responses to cancer models or treatments. The resulting profile supports comparisons between disease models when sampling and normalization are consistent.
Sample source matters because cytokines may be released by immune, tumor, or engineered cells, and the measured material can be a culture supernatant or blood sample. Standardized sampling reduces variation introduced during collection and handling, while normalization makes values more comparable across samples. These practices strengthen interpretation when comparing disease models or assessing treatment-related inflammation.
A typical workflow begins with collecting a defined culture supernatant or blood sample, followed by selection of an immunoassay suited to the cytokines being examined. Cytokine-specific antibodies capture and detect the targets, producing enzyme-based, fluorescent, or spot-based signals. Researchers then quantify the readout and apply consistent normalization before comparing cellular or treatment-related responses.
These methods differ mainly in how they represent cytokine detection. ELISA uses an enzyme-associated signal, multiplex bead-based assays use fluorescence to assess multiple cytokines, and ELISpot produces spots linked to cytokine-producing activity. The distinction affects the form of information collected, so researchers should match the assay format to whether they need selected measurements, multiple targets, or spot-based results.
Cytokine measurements help investigators characterize immune responses, evaluate immunotherapies and cell therapies, monitor treatment-related inflammation, and compare disease models. They can also examine activity associated with engineered cells alongside immune or tumor cells. By quantifying interferons, interleukins, tumor necrosis factor, or other selected factors, researchers obtain molecular readouts relevant to treatment and disease-model comparisons.