Ex Vivo Biodistribution
Ex Vivo Biodistribution - Related Videos
06:15
An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution
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2025
This video demonstrates an in vivo immunofluorescence localization (IVIL) technique to study the biodistribution of tumor-targeting antibodies in a murine model of breast cancer. Upon the intravenous injection of a tumor-specific antibody in the mouse, the tumor is surgically isolated, and the localization of the antibody within the tumor tissue is confirmed via ex vivo immunostaining.
13:29
Biodistribution of Nano-drug Carriers: Applications of SEM
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Nanoparticles have been increasingly used research towards targeted drug delivery and controlled drug release. While most of these particles have been developed as polymeric or liposomal particles because of their biocompatibility, there is a trend in current research toward the use of metallic and magnetic nanoparticles. These metallic nanoparticles...
08:53
In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research
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Cited by 1 •
2019
The in vivo immunofluorescence localization (IVIL) method can be used to examine in vivo biodistribution of antibodies and antibody conjugates for oncological purposes in living organisms using a combination of in vivo tumor targeting and ex vivo immunostaining methods.
13:35
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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Cited by 25 •
2017
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
14:04
Viral Nanoparticles for In vivo Tumor Imaging
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Cited by 45 •
2012
Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.
FAQs
Time points turn a single tissue measurement into a profile of distribution over time. Comparing organs and tumors at defined stages can show whether a product accumulates, is cleared, or shifts between tissues. This temporal view helps distinguish transient exposure from sustained presence, supporting interpretation of delivery performance and potential safety concerns in cancer studies.
Fluorescence and radiotracer detection provide measurement routes for locating and quantifying an administered agent in collected tissues. Their readouts support spatial comparisons among organs and tumors, allowing investigators to determine where the agent is detected and how tissue-associated signals differ. These measurements make distribution patterns assessable alongside accumulation and clearance.
Tissue-to-tissue differences reveal whether an agent preferentially reaches a tumor or also accumulates in non-target organs. This distinction connects distribution with two central questions: whether delivery supports tumor targeting and whether off-target exposure may affect safety. Comparing these patterns helps researchers evaluate the balance between intended localization and broader tissue exposure.
A typical workflow collects organs and tumor tissues at defined time points after administration, processes the samples, and measures agent-associated signals using fluorescence or radiotracer detection. Investigators then compare the resulting values across tissues and time points. The outcome is a spatial and quantitative record that supports assessment of accumulation, clearance, and tissue-to-tissue variation.
Researchers can apply the same tissue-level measurements to compare delivery systems according to where their agents accumulate and how those patterns change over time. In cancer research, this supports evaluation of nanoparticle formulations and antibody-based therapies for tumor targeting, while also revealing differences in off-target exposure. Such comparisons guide selection and optimization of delivery approaches.
Distribution data provide context for interpreting treatment outcomes by showing whether an agent reaches the tumor and where else it is present. A pattern of tumor accumulation may support the intended delivery strategy, whereas substantial signal in other tissues identifies exposure requiring consideration. These findings help optimize therapeutic agents, imaging probes, and biological products for cancer applications.