Fluorescent Tumor Imaging
Fluorescent Tumor Imaging - Related Videos
05:08
In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy
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Cited by 5 •
2013
In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.
09:04
In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
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Cited by 13 •
2014
We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.
03:10
In Vivo Fluorescence Imaging to Localize Antibodies in a Mouse Tumor Xenograft Model
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2025
The video demonstrates a fluorescence imaging method for in vivo antibody localization in a mouse tumor xenograft model. It involves the injection of tumor cells in a mouse to generate solid xenografts beneath the skin. Subsequent injection of fluorescent antibodies confirms specific localization through enhanced fluorescence signals within the tumor xenograft.
10:04
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
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Cited by 28 •
2018
We describe a protocol for preclinical in vivo tracking of cancer metastasis. It is based on a radionuclide-fluorescence reporter combining the sodium iodide symporter, detected by non-invasive [18F]tetrafluoroborate-PET, and a fluorescent protein for streamlined ex vivo confirmation. The method is applicable for preclinical in vivo cell tracking beyond tumor biology.
14:04
Viral Nanoparticles for In vivo Tumor Imaging
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Cited by 45 •
2012
Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.
FAQs
The probe’s biological distribution links fluorescence to a tumor target or tumor-associated feature. When it accumulates in or binds to that target, the resulting signal indicates where the feature is located within cells, tissues, or living models. This connection allows researchers to study tumor biology while relating a molecular event to visible spatial information.
Excitation light activates the probe, causing it to emit fluorescence that imaging systems can detect. Those systems capture the emitted light and convert it into spatial information, showing the location of labeled cancer cells, tissues, or tumor-associated features. The process therefore connects probe behavior at the molecular level with an image that can be analyzed.
High spatial resolution helps distinguish where fluorescent signals occur within cancer cells, tissues, or tumors. This localization supports more detailed assessment than a measurement that only indicates whether a signal is present. In research, spatially resolved fluorescence can connect tumor-associated features with their physical distribution, supporting tumor biology studies and evaluation of diagnostic or therapeutic strategies.
A fluorescent probe serves as the link between a selected molecular target and an observable signal. Once the probe accumulates in or binds to the target, excitation produces fluorescence at the associated location. Researchers can then examine that spatial signal to evaluate tumor-associated biology and to investigate strategies designed for cancer detection, diagnosis, or treatment.
A study first applies or introduces a fluorescent probe that can accumulate in or bind to a tumor target. The target-associated probe is then exposed to excitation light, and an imaging system captures the emitted fluorescence. Researchers interpret the resulting spatial information in cells, tissues, or living models to assess tumor features or experimental outcomes.
Researchers apply this approach when they need to visualize tumor-related features or follow cancer-associated changes spatially. Supported uses include tumor detection, surgical guidance, treatment-response monitoring, and investigation of tumor biology. The method also helps evaluate diagnostic and therapeutic strategies across cellular, tissue, and living-model studies by making molecularly associated signals visible.