Spontaneous Metastasis Mouse Model
Spontaneous Metastasis Mouse Model - Related Videos
08:11
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
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Cited by 83 •
2016
An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.
02:57
Spontaneous Metastasis Mouse Models: A Platform to Study the Metastatic Potential of Orthotopically Injected Colorectal Cancer Cells
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2023
This video describes the protocol to establish a spontaneous metastasis mouse model of colorectal cancer patient-derived xenografts or PDXs. These xenograft models partially mimic the features of human carcinomas and understand the critical aspects of human malignancies, including invasion and metastasis.
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
05:06
A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
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Cited by 2 •
2015
The metastatic spread of cancer is the major cause of cancer-related deaths. We provide an in-depth description of our survival surgery methodology for establishing a “patient-like” orthotopic syngeneic mouse model system for studying the mechanisms of metastasis in solid organ tumors.
07:43
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
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Cited by 32 •
2017
Brain metastasis has become an urgent unmet medical need as its incidence has increased while therapeutic options have remained palliative. Creating experimental animal models of brain metastasis via intracarotid arterial injection of cancer cells facilitates mechanistic studies of the disease biology and evaluation of novel intervention regimens.
FAQs
This model can retain sequential events that are often bypassed in other experimental designs, including local tumor growth, invasion, entry into blood or lymphatic vessels, survival in circulation, and colonization of distant organs. Preserving this sequence allows researchers to investigate metastatic progression as a connected biological process rather than examining only one isolated stage.
Introducing tumor cells into tissue that reflects their site of origin helps reproduce interactions between the tumor and its local microenvironment. Those interactions can influence primary growth, invasion, and subsequent dissemination. This tissue context therefore provides information that may be missed when cells are placed directly into the bloodstream, where local invasion and vascular entry are bypassed.
Direct bloodstream injection circumvents several early steps of metastatic progression, whereas the spontaneous approach retains local tumor development, invasion, and entry into blood or lymphatic vessels. The distinction matters because researchers can assess how the primary tumor and its surrounding environment contribute to dissemination, rather than focusing only on survival in circulation and colonization of distant organs.
Researchers typically introduce tumor cells into a tissue corresponding to the tumor’s site of origin, allowing a primary tumor to develop in vivo. The resulting system is then used to study progression from local disease through dissemination and distant colonization. This arrangement supports evaluation of both the primary tumor and metastases within one connected experimental model.
It can help identify determinants of metastatic progression and examine how tumors interact with their microenvironment during dissemination. Because the model encompasses local disease and distant spread, researchers can relate primary tumor behavior to later metastatic outcomes. This broader perspective supports investigation of why tumor cells progress through the metastatic cascade and establish disease in secondary organs.
The model is useful when investigators need to evaluate therapies against both primary disease and established metastases. Its in vivo progression incorporates the development of a primary tumor followed by distant dissemination, allowing treatment effects to be considered across multiple stages of cancer progression. This makes it relevant to cancer research focused on therapeutic control of metastatic disease.