Kidney Extraction
Kidney Extraction - Related Videos
03:00
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
0 Views •
2023
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
09:15
Reliable and High Efficiency Extraction of Kidney Immune Cells
0 Views •
Cited by 12 •
2016
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
08:19
The Mouse Isolated Perfused Kidney Technique
0 Views •
Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
01:45
Kidney Structure
0 Views •
2019
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries. The renal cortex is the thick outer layer of the kidney. It...
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
0 Views •
2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
FAQs
Preservation maintains the physical and biological material needed for downstream examination. Intact tissue can support histological analysis, while preserved cellular material can be used for genomic or biochemical studies. In cancer research, careful handling helps ensure that observed tissue structure, molecular changes, and biochemical findings reflect the kidney’s condition rather than damage introduced during removal or processing.
The vessels and ureter are key structures that must be identified and isolated from the kidney before removal. Addressing these connections allows the organ to be separated from surrounding anatomy while preserving the tissue or cellular material required for analysis. Their careful handling supports more complete specimens and helps maintain the anatomical context needed for interpreting renal changes.
An intact kidney permits analysis at several biological levels within the same organ. Researchers can examine overall tissue organization, tumor formation, metastasis, and tissue damage, then relate those findings to genomic or biochemical changes. This combination connects organ-level pathology with molecular evidence, providing a broader picture of cancer progression than cellular or molecular analysis alone.
The procedure requires coordinated access to the abdominal cavity, identification of the kidney, isolation from surrounding tissues, separation of its vessels and ureter, and removal of the organ. Handling and preservation follow immediately because the specimen may be needed for histological, genomic, or biochemical analysis. Each stage contributes to obtaining usable renal tissue for clinical or laboratory purposes.
Extracted kidneys are useful when investigators need to evaluate tumor formation, metastasis, tissue damage, molecular changes, or responses to experimental therapies. The specimen provides material for examining how cancer affects the organ and how treatment alters those effects. This makes extraction relevant to studies that connect structural findings with molecular and biochemical outcomes.
An extracted kidney can provide histological findings about tissue structure, genomic information about molecular changes, and biochemical measurements associated with disease or treatment. These data can be examined alongside evidence of tumor formation, metastasis, or tissue damage. Together, the results help researchers assess cancer progression and determine how experimental therapies affect renal tissue.