Tumorigenesis
Tumorigenesis - Related Videos
07:45
Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis
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2024
The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T cells.
03:26
Chicken Chorioallantoic Membrane-based Cancer Modeling: An In Ovo Model to Study Cancer Cell Tumorigenesis and Metastasis
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2023
In this video, we describe the implantation of cancer cells onto a chicken chorioallantoic membrane to generate an in ovo model. The model successfully engrafts the ovarian cancer cells to study the progression of gynecological cancer.
09:37
Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
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2021
Here, we present a protocol for performing gene knockouts that are embryonic lethal in vivo in genetically engineered mouse model-derived tumors and then assessing the effect that the knockout has on tumor growth, proliferation, survival, migration, invasion, and the transcriptome in vitro and in vivo.
04:38
Intracardiac Delivery of Viral Vectors: A Technique to Inject Viral Vectors Intracardially to Study Pancreatic Metastasis in Murine Model
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2023
This video describes the protocol for intracardiac injection of retroviruses into the transgenic mouse to ensure gene transfer into proliferating pancreatic β premalignant lesions. This method can also help in assessing the functions of candidate genes in tumor metastasis.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
FAQs
Genetic changes can disrupt regulators of cell division, DNA repair, and apoptosis, while epigenetic changes can also alter how relevant cellular programs operate. These disturbances may give affected cells abnormal growth and survival properties. Examining both types of alteration helps cancer researchers connect early molecular events with later tumor formation and identify changes that contribute to disease development.
The multistep character matters because tumor formation and progression are associated with additional alterations, not a single isolated event. Early disruptions can be followed by changes that support angiogenesis, immune evasion, invasion, or metastasis. Tracking this accumulation helps researchers study how tumors evolve over time and why different tumors may display different biological behavior.
Angiogenesis can support tumor progression, whereas immune evasion can reduce effective interaction with immune defenses. Invasion enables tumor cells to move into surrounding tissues, and metastasis extends disease to other sites. Studying these properties allows cancer researchers to distinguish mechanisms associated with local progression from those linked to spread, providing important context for understanding advanced disease.
Researchers examine risk factors and driver mutations to identify events associated with disease initiation. This work connects relevant influences and molecular alterations with the earliest stages of tumor development. Clarifying their roles can guide biomarker development and early detection research, while also identifying biological events that may be useful for prevention strategies or therapeutic investigation.
Tumor evolution is associated with different combinations of genetic or epigenetic alterations and progression-related properties. Consequently, cancers can differ in the mechanisms governing growth, survival, angiogenesis, immune evasion, invasion, and metastasis. Cancer researchers study these differences to explain variable treatment responses and to inform targeted therapies or combinations designed around the biology of a tumor.
By connecting molecular changes with tumor formation and progression, tumorigenesis research identifies processes that may serve as biomarkers for disease detection or progression assessment. The same knowledge can reveal opportunities for prevention, clarify why cancers behave differently, and support the design of targeted or combination therapies. These applications translate mechanistic findings into strategies for managing cancer.