Desorption
Desorption - Related Videos
01:08
Matrix-Assisted Laser Desorption Ionization (MALDI)
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2025
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Cited by 19 •
2016
A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.
06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Cited by 21 •
2013
Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue sections.
09:38
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Cited by 3 •
2013
Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.
07:53
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
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Cited by 5 •
2020
Neutral SO2 clusters of low kinetic energy (< 0.8 eV/constituent) are used to desorb complex surface molecules such as peptides or lipids for further analysis by means of mass spectrometry using an ion trap mass spectrometer. No special sample preparation is required, and real-time observation of reactions is possible.
FAQs
Several triggers can promote desorption, but they act by weakening surface interactions in different ways. Heating supplies thermal energy, pressure changes alter the surrounding environment, and solvent interactions can favor transfer into a liquid or solution. Light or a chemical reaction can also change the surface or attached species. The relevant trigger depends on the material and the species being released.
Temperature-programmed desorption uses a controlled heating sequence to connect release behavior with surface binding. As temperature changes, the material releases retained species, and the resulting pattern provides evidence about binding strength and surface coverage. This makes the approach useful for comparing catalyst or adsorbent surfaces, especially when retention properties need to be related to material performance.
Desorption kinetics describe how quickly a species leaves a surface, whereas equilibrium describes the balance between retention and release under specified conditions. Considering both is important because a material may capture a species effectively yet release it slowly, or respond strongly to a change in temperature, pressure, or solvent. These distinctions help predict storage, separation, and contaminant-removal behavior.
The surrounding phase influences where a released species can go and which interactions remain favorable. Desorption into a gas differs from release into a liquid or solution because pressure and solvent interactions affect the balance at the interface. This distinction matters when choosing conditions for a separation, evaluating an adsorbent, or predicting whether a captured species will remain retained.
In chromatography, desorption helps control when compounds leave a surface and continue through the separation system. Adjusting the conditions that weaken surface interactions can therefore influence release and the resulting separation behavior. The same principle applies more broadly to chemical separations, where selective retention followed by controlled release determines how effectively different species can be handled.
For gas storage, the balance between capture and release is central to whether a material can load a gas and later deliver it. Desorption conditions such as temperature or pressure changes can influence that release. Studying this response helps assess how adsorbent and porous materials retain stored species and how controllably the stored gas can be recovered.
Environmental contaminant removal depends on more than initial capture. Desorption analysis shows how strongly contaminants are retained and whether changed conditions could promote their release from an adsorbent. Measuring or interpreting this behavior helps distinguish temporary retention from more persistent capture, supporting evaluation of materials intended to remove contaminants from environmental systems.