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Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

DOI:
10.3791/60487-v

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07:53 min

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March 01, 2020

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, , , ,
1Institute of Applied Physics and Center for Materials Research (LaMa),Justus Liebig University Giessen, 2Bruker Daltonik GmbH

Chapters

  • 00:04Introduction
  • 01:35Preparation of Substrates and Samples
  • 03:34Preparation of DINeC Mass Spectrometer
  • 04:48Measurement of Mass Spectra
  • 06:13Results: Real-time Observation of Thermal Degradation of Angiotensin II
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Neutral SO2 clusters of low kinetic energy (< 0.8 eV/constituent) are used to desorb complex surface molecules such as peptides or lipids for further analysis by means of mass spectrometry using an ion trap mass spectrometer. No special sample preparation is required, and real-time observation of reactions is possible.

Tags

Keywords: Cluster-induced Desorption/ionizationMass SpectrometryDINeCBiomoleculesPeptidesProteinsIsotope DipeptideSO2 ClustersSupersonic ExpansionIon TrapSilicon SubstratesHighlighter InkAngiotensin IIVacuum System

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