Spe Cartridge
Spe Cartridge - Related Videos
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
0 Views •
2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
09:49
Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency
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Cited by 7 •
2016
Endocrine Disrupting Compounds (EDC) pose a substantial risk to the aquatic environment. Municipal wastewater treatment plants are major contributors to the estrogenic potency of surface waters. The methodology provided in this paper allows for an assessment of the efficacy and suitability of wastewater treatment processes with respect to EDC removal.
10:24
Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
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2024
We demonstrate a medium-throughput method for quantification of aflatoxins and stilbenoid phytoalexins in single peanut seeds using ultra performance liquid chromatography. This method was specifically developed for the analyses of wild Arachis species challenged by the aflatoxigenic Aspergillus species.
11:12
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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Cited by 8 •
2022
The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.
FAQs
Retention depends on the interaction between the analyte and the packed sorbent. Adsorption and partitioning separate compounds through their association with the sorbent phase, whereas ion exchange uses charge-related interactions and affinity relies on selective molecular recognition. These mechanisms determine which compounds remain during sample processing and which are removed or recovered during elution.
The sorbent determines which chemical interactions dominate inside the cartridge, so it strongly influences both selectivity and recovery. A suitable material retains the target compounds while allowing unwanted matrix components to be removed during washing. If the interaction is poorly matched to the sample, target compounds may not be retained effectively or may be difficult to elute.
Solvent conditions regulate how strongly analytes interact with the sorbent and whether they remain retained or move into the collected fraction. During washing, conditions should favor removal of interfering matrix components without substantial analyte loss. During elution, a suitable solvent weakens the retention interaction sufficiently to release the targets for analysis.
Washing is a selective cleanup stage. It removes matrix components that remain in the liquid or interact less strongly with the sorbent, while the target compounds stay retained through the chosen extraction mechanism. Effective washing therefore improves sample purity and reduces substances that could interfere with subsequent chemical measurements.
The sample is passed through the cartridge so target compounds can interact with the packed sorbent. A washing step removes unwanted matrix constituents while the analytes remain retained. Finally, a suitable solvent elutes the retained compounds, and the resulting fraction is collected for chemical analysis. Sorbent and solvent choices govern the outcome.
Chemists use this preparation approach when complex liquid samples contain interfering substances or when target compounds need to be concentrated before measurement. The cartridge can improve sample purity and support sensitive, reproducible analysis by chromatography or mass spectrometry. Its value is greatest when selective retention and controlled elution produce a cleaner analytical fraction.