Leak Test
Leak Test - Related Videos
06:39
An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse
0 Views •
Cited by 68 •
2018
In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.
06:59
Intrathecal Application of a Fluorescent Dye for the Identification of Cerebrospinal Fluid Leaks in Cochlear Malformation
0 Views •
Cited by 2 •
2020
Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital microscope.
13:40
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
0 Views •
Cited by 17 •
2011
The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.
08:29
Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
0 Views •
Cited by 5 •
2018
Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.
12:03
Viability Assays for Cells in Culture
0 Views •
Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
FAQs
Pressure or vacuum testing creates a measurable starting condition inside or around the item being evaluated. If containment is compromised, the measured pressure can change during the observation period. Monitoring that change over time provides an integrity signal, making loss of containment assessable in enclosed pathways, packages, and devices used in clinical settings.
Tracer-based methods use a different detection principle: instead of inferring a defect from pressure behavior, they identify escape of a gas or liquid. This provides direct evidence that material has crossed a boundary. Pressure, vacuum, gas, and liquid approaches therefore offer alternative ways to examine integrity across clinical components and fluid pathways.
The test condition must be controlled because the result depends on the applied pressure or vacuum and the time over which the system is monitored. A pressure change is meaningful only in relation to that defined starting condition and observation period. Consistent control supports comparisons among components, packages, and fluid pathways during quality-control evaluation.
A basic workflow begins by connecting the component, package, or fluid pathway to a test setup, applying a controlled pressure or vacuum, and observing the response over time. Where appropriate, the workflow instead uses a tracer gas or liquid and monitors whether it escapes. The resulting signal is then used to assess integrity before clinical use.
In clinical manufacturing and quality control, leak testing can be applied to sterile medical packaging, tubing, catheters, syringes, and other devices designed to contain fluids or gases. The suitable approach depends on whether the item is best evaluated through pressure behavior or detection of escaping material, supporting integrity checks across different device formats.
An unfavorable result can reveal a manufacturing defect or a failure to maintain containment, prompting evaluation before the item reaches clinical use. For sterile packaging and fluid-handling devices, identifying such problems helps reduce contamination risk and supports confirmation that the product performs its intended containment function. The test therefore contributes to quality assurance and safety.