In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.
Wick, M. J., Harral, J. W., Loomis, Z. L., Dempsey, E. C. An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse. J. Vis. Exp. (139), e57037, doi:10.3791/57037 (2018).