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An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse
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An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

DOI:
10.3791/57037-v

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06:39 min

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September 12, 2018

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, , ,
1Cardiovascular Pulmonary Research Laboratory,University of Colorado Denver, 2Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado Denver, 3Denver VA Medical Center

Chapters

  • 00:04Title
  • 00:59Catheter Insertion
  • 03:12Injections and Organ Isolation
  • 04:50Results: Representative Substance P-induced Extravasation Analyses
  • 05:49Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.

Tags

Keywords: Evans Blue ProtocolVascular LeakPlasma ExtravasationMouseCatheterizationJugular VeinEvans Blue DyeVascular FieldOrgan Of InterestSurgical TechniqueHeparinized Saline

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