An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

Marilee J. Wick; Julie W. Harral; Zoe L. Loomis; Edward C. Dempsey

doi:10.3791/57037

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Medicine

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An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

JoVE Journal
Medicine

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JoVE Journal Medicine

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

26,450 Views

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06:39 min

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September 12, 2018

DOI:

10.3791/57037-v

DOI:

10.3791/57037-v

•

06:39 min

September 12, 2018

•

26405 Views

Marilee J. Wick^1,2, Julie W. Harral^1,2, Zoe L. Loomis^1,2, Edward C. Dempsey^1,2,3

¹Cardiovascular Pulmonary Research Laboratory,University of Colorado Denver ²Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado Denver ³Denver VA Medical Center

Summary

In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.