Apolipoprotein B Assay
Apolipoprotein B Assay - Related Videos
07:08
A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
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Cited by 3 •
2016
We describe a high-throughput, multiplex, and targeted proteomic cerebrospinal fluid (CSF) assay developed with potential for clinical translation. The test can quantitate potential markers and risk factors for neurodegeneration, such as the apolipoprotein E variants (E2, E3 and E4), and measure their allelic expression.
07:37
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
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Cited by 2 •
2020
Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.
10:59
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Cited by 32 •
2013
Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.
07:36
Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
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Cited by 8 •
2018
We describe a protocol to induce atherosclerosis in the aortic root of ApoE-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.
09:14
Methods to Assay Drosophila Behavior
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Cited by 259 •
2012
Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.
FAQs
The assay converts selective recognition into a concentration estimate. Antibodies bind apolipoprotein B in the biological sample, producing an immunochemical signal. That signal is not interpreted in isolation: it is compared with calibrated standards, allowing the measured response to be related to apolipoprotein B concentration. This design links molecular binding to a quantitative readout for lipid-transport studies.
Because apolipoprotein B is the primary structural protein of atherogenic lipoprotein particles, its measurement can help researchers examine changes associated with lipoprotein transport and assembly. The assay therefore contributes information about lipid-related biology beyond a general observation of metabolic change. In developmental studies, that information can be compared across growth or differentiation states to track changing lipid metabolism.
Immunoturbidimetric detection and other immunochemical formats share the same central recognition event but differ in how the antibody interaction becomes measurable. Immunoturbidimetry is one commonly identified signal strategy, whereas the broader immunochemical category includes other detection approaches. This distinction matters when comparing assays because the detection format determines how antibody binding is converted into the signal used for calibration.
Calibrated standards are essential because they provide the reference needed to translate an observed signal into concentration. Without that comparison, an antibody-generated signal would remain a relative measurement rather than a quantified result. Standards make results interpretable across samples within the assay and support comparisons of apolipoprotein B-associated changes in cells, tissues, or organisms.
A basic workflow begins with a biological sample, exposes it to antibodies directed against apolipoprotein B, measures the resulting immunochemical signal, and relates that response to calibrated standards. The output is a concentration estimate rather than merely a record of antibody binding. This sequence provides a consistent framework for examining lipid transport, lipoprotein assembly, or metabolic change.
The appropriate biological material depends on the developmental question. The assay can be applied to cells, tissues, or organisms, allowing investigators to examine lipid metabolism at different biological levels. Measurements from these contexts can help connect cellular or tissue changes with broader organismal patterns during development or disease-related developmental processes.
In developmental biology, measurements can be organized around transitions such as growth and differentiation rather than treated as isolated lipid values. Comparing apolipoprotein B concentrations across those states may reveal how lipoprotein assembly or lipid transport changes as development progresses. The same approach can support analysis of disease-related developmental processes, where metabolic patterns may shift alongside developmental change.