Birefringence Analysis
Birefringence Analysis - Related Videos
08:01
Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays
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Cited by 93 •
2013
The zebrafish is now an established and powerful tool for modeling muscular dystrophies, congenital myopathies, and related neuromuscular diseases. Birefringence and touch-evoked escape behavior are two common noninvasive assays used to determine the degree of muscular disorganization and locomotive impairment of zebrafish embryos during early development.
07:03
In Situ Measurement of Vacuum Window Birefringence using 25Mg+ Fluorescence
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2020
Presented here is a method to measure the birefringence of vacuum windows by maximizing the fluorescence counts emitted by Doppler cooled 25Mg+ ions in an ion trap. The birefringence of vacuum windows will change the polarization states of the laser, which can be compensated by changing the azimuthal angles of external wave plates.
09:38
Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies
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Cited by 1 •
2018
Fabrication procedures for highly magnetically responsive lanthanide ion chelating polymolecular assemblies are presented. The magnetic response is dictated by the assembly size, which is tailored by extrusion through nanopore membranes. The assemblies' magnetic alignability and temperature-induced structural changes are monitored by birefringence measurements, a complimentary technique to nuclear magnetic resonance and small angle neutron scattering.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
10:39
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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Cited by 3 •
2022
Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
FAQs
Retardance arises when anisotropic components split polarized light into waves that travel at different speeds. The resulting phase shift reflects directional differences in the specimen’s optical behavior. Because structural order influences these differences, measuring retardance can provide an optical signature of organization within cellular or extracellular components rather than simply indicating that biological material is present.
After polarized light passes through the specimen, the analyzer records changes produced by the phase relationship between the separated light waves. Those changes can appear as differences in intensity or color. These optical signals make otherwise subtle structural order detectable and allow researchers to examine spatial organization without relying only on direct visual identification of the underlying components.
Directional organization affects how a specimen alters polarized light, so aligned cellular or extracellular structures can generate measurable optical signatures. In developmental biology, those signatures can be related to morphogenesis, cytoskeletal arrangement, and tissue maturation. The analysis therefore helps connect structural orientation with developmental state, while changes in the signal may indicate differences in tissue organization.
The measurement begins by placing the specimen in a polarized-light pathway and passing the light through the developing material. An anisotropic response produces separated waves and a phase shift, which an analyzer records as intensity or color changes. Researchers can then quantify the birefringence-related signal and compare optical patterns across structures, developmental stages, or experimental conditions.
It is useful when researchers need information about structural organization in embryos or developing tissues without damaging the specimen. Birefringence Analysis can reveal patterns associated with morphogenesis, cytoskeletal arrangement, and tissue maturation. Because the method provides optical rather than destructive structural information, it supports comparisons of developmental organization across specimens or conditions.
Quantified optical signatures provide a basis for comparing the organization of cellular or extracellular structures between normally developing material and experimentally altered samples. Differences in measured birefringence can indicate changes in structural order or alignment. Interpreted alongside developmental observations, these comparisons help assess how an experimental condition affects morphogenesis, cytoskeletal arrangement, or tissue maturation.