Live Zebrafish Analysis
Live Zebrafish Analysis - Related Videos
08:01
Induction of Hypoxia in Living Frog and Zebrafish Embryos
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Cited by 5 •
2017
We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.
09:10
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
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Cited by 5 •
2010
Multiphoton microscopy allows control of low energy photons with deep optical penetration and reduced phototoxicity. We describe the use of this technology for live cell labeling in zebrafish embryos. This protocol can be readily adapted for photo-induction of various light-responsive molecules.
10:10
Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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Cited by 8 •
2016
Mitosis is critical to every living organism and defects often lead to cancer and developmental disorders. Using this imaging protocol and zebrafish as a model system, researchers can visualize mitosis in a live vertebrate organism and the multitude of defects that arise when mitotic processes are defective.
09:39
Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
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Cited by 20 •
2011
Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.
11:05
Analysis of Oxidative Stress in Zebrafish Embryos
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Cited by 97 •
2014
Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.
FAQs
Fluorescent reporter lines make selected biological activity visible during imaging by producing a fluorescent signal associated with the process or tissue being studied. In a living embryo or larva, researchers can follow that signal over time rather than relying only on a fixed endpoint. This links changes in gene expression or cell behavior with subsequent tissue and organ development.
Time-lapse imaging records successive views of the same living specimen, allowing researchers to follow changes rather than infer them from one fixed stage. This can reveal the sequence and timing of cell movements, tissue formation, gene-expression changes, and organ development. The resulting record helps relate an early event to a later developmental outcome.
Together, these features make internal developmental events accessible while the embryo or larva remains alive. Researchers can visualize cells, tissues, fluorescent signals, and forming organs without relying on fixation or physical sectioning. That preserves a continuous view of development and supports direct comparison between observed dynamics and later structural outcomes.
These microscopy approaches provide ways to visualize living embryos or larvae while tracking developmental features over time. Used with fluorescent reporter lines, they can help researchers follow gene expression, cell movements, tissue formation, and organ development. The resulting images connect molecular or cellular signals with visible changes in the developing specimen.
Researchers image living zebrafish embryos or larvae with a microscopy approach, often using fluorescent reporter lines and time-lapse acquisition. They then track features such as cell movements, tissue formation, gene expression, or organ development across the recorded period. Interpretation connects these changing signals or structures with developmental processes and any genetic or chemical perturbation being examined.
Researchers choose it when they need to observe embryogenesis, regeneration, disease modeling, or responses to genetic and chemical perturbations in living specimens. The approach can show how molecular or cellular changes unfold into visible developmental outcomes. Its use therefore extends beyond describing anatomy, supporting experiments that connect biological mechanisms with observable changes over time.