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Analysis of Oxidative Stress in Zebrafish Embryos
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of Oxidative Stress in Zebrafish Embryos
DOI:

11:05 min

July 07, 2014

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Chapters

  • 00:05Title
  • 01:54Preparations for Oxidative Stress Induction and ROS-detection
  • 03:01Oxidative Stress Induction
  • 04:28Whole Mount ROS-detection
  • 05:56Single Cell ROS-detection
  • 09:08ROS-detection in Whole Mount and Single Cells
  • 10:30Conclusion

Summary

Automatic Translation

Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.

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