Sac Integrity
Sac Integrity - Related Videos
10:38
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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Cited by 19 •
2008
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
04:34
Surgical Treatment of an Endolymphatic Sac Tumor
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Cited by 1 •
2023
Here, we discuss clinical and radiological features of endolymphatic sac tumors and report the methodology and results of a surgical removal in a case in care at our otologic referral center.
11:53
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Cited by 6 •
2016
To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.
04:34
Dissection of the Endolymphatic Sac from Mice
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Cited by 5 •
2021
This study describes how to dissect the endolymphatic sac from the inner ear of the mouse at different ages. The result of a similar dissection is shown in an Slc26a4-null mouse model of enlargement of the endolymphatic sac. A transgenic mouse with a fluorescent reporter expressed in the endolymphatic sac is presented as a model to readily visualize the endolymphatic sac, and educational tool.
06:04
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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Cited by 41 •
2016
This protocol describes the use of excised intestinal tissue preparations or "intestinal sacs" as an ex vivo model of intestinal barrier function. This model may be used to assess integrity of both the epithelial barrier and the mucous gel layer at specific intestinal sites in animal models of digestive disease.
FAQs
Sac Integrity depends on several interacting supports rather than a single structural feature. Cell adhesion and intercellular junctions maintain connections between neighboring cells, while the extracellular matrix provides additional tissue support. Regulation of internal fluid pressure limits mechanical stress on the enclosed structure. Together, these mechanisms help preserve continuity, retain contents, and maintain compartment organization during development.
Internal fluid pressure can influence the mechanical load placed on a developing sac. If pressure is not adequately regulated, the structure may become more vulnerable to leakage, tearing, or disruption of its enclosed compartment. Assessing pressure-related effects therefore connects structural observations with tissue mechanics and can help explain why otherwise organized tissues lose stability during development.
Disruption of cell adhesion, intercellular junctions, or extracellular-matrix support can reduce the coordinated reinforcement needed for a stable sac. These changes may compromise the continuity of the tissue and interfere with its barrier function. Studying the contributions of each component helps distinguish whether a developmental defect reflects weakened cell connections, insufficient matrix support, or broader tissue instability.
Morphogenesis requires tissues to maintain organized structures while they change shape and position. Loss of Sac Integrity can reveal that this remodeling has exceeded the tissue’s ability to preserve adhesion, junctional continuity, matrix support, or pressure control. For developmental biologists, such changes provide structural evidence linking abnormal tissue mechanics with failures in embryonic or extraembryonic organization.
Evaluation can show whether embryonic or extraembryonic tissues have maintained structural stability during development. Researchers can use these observations to monitor developmental viability and identify defects associated with genetic, chemical, or physical perturbations. The assessment is especially informative when interpreted alongside tissue organization, because a compromised sac may indicate broader problems in formation or maintenance.
Comparing sac structure after genetic, chemical, or physical perturbation can reveal whether an intervention is associated with leakage, tearing, or loss of compartmental organization. These outcomes provide a readout of tissue stability rather than only of cell-level changes. Such comparisons help connect the type of perturbation with altered adhesion, matrix support, pressure regulation, or developmental viability.
Both embryonic and extraembryonic tissues depend on organized compartments and stable barriers during development. Examining Sac Integrity in these contexts helps researchers evaluate whether tissue formation proceeds with adequate structural support and containment. The resulting observations contribute to studies of morphogenesis, tissue mechanics, developmental viability, and the structural consequences of genetic, chemical, or physical disruption.