T Cell Receptor Repertoire
T Cell Receptor Repertoire - Related Videos
08:59
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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Cited by 3 •
2021
The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.
04:24
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
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Cited by 3 •
2011
We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.
10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
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Cited by 21 •
2010
We present a protocol to produce antigen-specific mouse T-cells using retroviral...
01:28
Types of Receptors: Cell Surface Receptors
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2023
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
08:51
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
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Cited by 4 •
2019
Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.
FAQs
V(D)J recombination assembles different combinations of variable, diversity, and joining gene segments during T cell development. Each assembly can produce a distinct receptor sequence, allowing the developing T cell population to recognize many molecular targets. This gene-segment recombination provides the foundational diversity on which later junctional variation and thymic selection act.
Junctional changes introduce additional sequence variation at the points where receptor gene segments join. This process increases the number of distinct receptor sequences beyond the combinations created by segment selection alone. As a result, developing T cells can generate a broader range of antigen-recognition possibilities before thymic selection determines which receptors remain in the mature pool.
Positive and negative selection impose complementary filters on developing T cells. Together, they determine which receptors enter the mature T cell pool while contributing to self-tolerance. Their combined action balances the preservation of broad antigen recognition with the removal or restriction of receptor specificities that could be problematic for recognition of self.
T cell receptor repertoire analysis can reveal how receptor diversity changes as developing lymphocytes pass through thymic selection. Patterns in the repertoire help investigators examine how positive and negative selection shape the mature pool and how developing cells balance broad antigen recognition with self-tolerance. This makes repertoire analysis a way to study selection outcomes rather than receptor generation alone.
Infection and vaccination research can use repertoire analysis to examine changes in T cell receptor patterns associated with immune responses. The approach helps connect receptor-pool changes with immune system activity without limiting investigation to a single receptor. These comparisons can clarify how immune recognition is altered as the organism responds to infectious or vaccine-related challenges.
The repertoire provides a readout of several linked developmental processes: receptor gene assembly, junctional diversification, thymic selection, and immune system maturation. Studying these relationships helps developmental biologists investigate how a functional T cell population emerges while maintaining self-tolerance. The same framework also supports research into changes associated with autoimmunity and immune-based therapies.