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JoVE Journal
Immunology and Infection

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
 

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Article DOI: 10.3791/61792-v
January 12th, 2021

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Summary January 12th, 2021

The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

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