Thy1-yfp Mouse
Thy1-yfp Mouse - Related Videos
10:03
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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Cited by 11 •
2012
Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.
07:51
Intravital Two-Photon Imaging of Touch Sensory Axon Morphology in Mouse Skin
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2025
This study presents an in vivo two-photon imaging approach for visualizing the structures of low-threshold mechanoreceptor (LTMR) axon terminals in the forepaw skin of mice. Using repeated, high-resolution imaging of individual axons, this method provides a new platform for studying the structure and function of the cutaneous sensory circuit during development and in adults.
07:04
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
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Cited by 2 •
2019
We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy in vivo.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
10:28
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Cited by 11 •
2016
Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.
FAQs
Thy1-YFP expression is governed by regulatory elements from the Thy1 gene, so fluorescence appears in selected subsets of differentiated neurons rather than uniformly across every neural cell. This expression pattern makes the model useful for examining how particular labeled neurons change during development, while recognizing that unlabeled neurons are not directly visualized.
Fluorescence can reveal cell bodies, axons, dendrites, and synaptic processes within labeled neurons. Examining these structures together allows developmental biologists to connect changes in neuronal form with processes such as axon growth, dendritic remodeling, and circuit formation. The model therefore supports structural analysis at several levels of neuronal organization.
Selective labeling provides a way to follow the morphology of particular differentiated neurons as the nervous system develops. Rather than treating neuronal development only as a population-level process, investigators can relate visible changes in axons, dendrites, and synaptic processes to neuronal migration and circuit formation. This links cellular structure with developmental progression.
Investigators examine intact tissues with fluorescence microscopy to locate YFP-labeled neuronal structures and document their morphology. Longitudinal or live imaging approaches can extend this analysis across developmental stages or observation periods, allowing structural changes to be followed over time. The resulting images support comparisons of neuronal growth, remodeling, and organization.
The model supports visualization of neuronal migration, axon growth, dendritic remodeling, and circuit formation. These observations help researchers track how neuronal structures change as developmental organization proceeds. Because fluorescence marks multiple parts of selected neurons, studies can relate movement and outgrowth to later changes in neuronal architecture and emerging connections.
Thy1-YFP mice help assess structural changes in neurodevelopmental disease and injury by making selected neuronal processes visible for fluorescence-based analysis. Researchers can examine alterations in cell bodies, axons, dendrites, or synaptic processes and relate those findings to broader changes in neuronal morphology. This provides a structural context for developmental abnormalities and damage-related remodeling.