Zebrafish Model Studies
Zebrafish Model Studies - Related Videos
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
08:13
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish
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Cited by 7 •
2021
We modified the Marmarou weight drop model for adult zebrafish to examine a breadth of pathologies following blunt-force traumatic brain injury (TBI) and the mechanisms underlying subsequent neuronal regeneration. This blunt-force TBI model is scalable, induces a mild, moderate, or severe TBI, and recapitulates injury heterogeneity observed in human TBI.
09:30
Methods for the Study of the Zebrafish Maxillary Barbel
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Cited by 7 •
2009
The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.
10:42
Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
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Cited by 7 •
2016
Sustained fibrosis with deposition of excessive extracellular matrix proteins leads to cirrhosis. Alcohol abuse is one of the main causes of severe liver disease. We established an ethanol-induced zebrafish fibrotic liver model to study the mechanisms and strategies of promoting hepatocyte regeneration upon alcohol-induced injury.
08:59
Using the optokinetic response to study visual function of zebrafish
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Cited by 34 •
2010
Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.
FAQs
Signaling pathways regulate development by influencing cell fate and tissue patterning, the processes that determine which cells adopt particular identities and how groups of cells become organized. In zebrafish embryos, researchers can connect changes in gene function with visible developmental outcomes, helping identify mechanisms that control organ formation and embryonic development.
Zebrafish embryos provide two experimental advantages for developmental observation: they develop outside the mother and remain transparent. This allows researchers to follow organ formation directly with live imaging. Observing development over time can reveal when a defect emerges and relate it to earlier changes in patterning or cell fate.
Targeted mutations alter gene function, whereas controlled exposure to experimental compounds tests how those compounds affect developmental outcomes. Researchers can compare these perturbations with normal embryonic development to determine whether a gene or compound changes signaling, cell fate, tissue patterning, or organ formation. This connects a specific intervention with observable consequences during vertebrate development.
A typical investigation combines embryos with one or more complementary observations. Researchers may introduce a targeted genetic change or apply an experimental compound under controlled conditions, then use live imaging to monitor development. They interpret these observations alongside changes in gene function and visible organ formation, relating an intervention to cell fate, tissue patterning, or embryonic development.
Zebrafish model studies can investigate mechanisms relevant to human congenital disorders by showing how altered gene function or signaling affects embryonic development. Because researchers can observe these effects during organ formation, the model supports a mechanistic link between early developmental changes and disease-related outcomes. Findings may also guide therapeutic research by identifying biological processes for further investigation.
Findings from zebrafish support comparative analyses of vertebrate development by providing evidence from a vertebrate organism in which researchers can directly observe and experimentally manipulate embryonic processes. Results can clarify general principles of tissue patterning and organ formation while helping assess whether developmental mechanisms may be relevant to human congenital disorders and therapeutic research.