Chemical Imaging

Chemical imaging is the spatially resolved detection and mapping of chemical composition, revealing where specific molecules, elements, or materials occur within a sample. It works by collecting position-dependent signals from techniques such as infrared or Raman spectroscopy, fluorescence microscopy, or mass spectrometry, then converting spectral or intensity data into chemical maps. In engineering, these maps help characterize materials, interfaces, coatings, microstructures, and manufacturing processes without relying only on bulk measurements. Chemical imaging can identify contaminants, degradation, defects, and compositional variation, supporting quality control, failure analysis, process optimization, and the design of advanced materials and devices.

Chemical Imaging - Related Videos

Research

JoVE Journal - Biology
Free Sample

Development of automated imaging and analysis for zebrafish chemical screens.

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Cited by 48 •

2010

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

Research

JoVE Journal - Chemistry

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

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Cited by 2 •

2022

Here, we present a protocol for high-resolution optical detection of chemical information around implanted medical devices with X-ray excited luminescence chemical imaging (XELCI). This novel imaging technique is developed in our lab which enables studying implant-associated infection biochemistry.

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

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Cited by 14 •

2015

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

Education

JoVE Core - Chemistry

Chemical Formulas

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2020

A chemical formula presents information about the proportions of atoms constituting a particular chemical compound or molecule, mainly using symbols of elements and numbers. At times other symbols, such as dashes, parentheses, brackets, commas, plus, and minus signs, are also used. A chemical formula can be one of three types – molecular, empirical, and structural. Molecular Formula The molecular formula represents a molecule or a chemical compound using chemical symbols (to indicate the...

Chemical Equations

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2020

Chemical equations represent the identities and relative quantities of substances involved in a chemical reaction. The substances undergoing reaction are called reactants, and their formulas are placed on the left side of the equation. The substances generated by the reaction are called products, and their formulas are placed on the right side of the equation. Plus signs (+) separate individual reactant and product formulas, and an arrow (→) separates the reactant and product (left and right)...

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