Portable Sequencer
Portable Sequencer - Related Videos
08:57
Amplicon Sequencing using the Long-Read Sequencing Technologies
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2025
This protocol was optimized for targeted deep sequencing of 18 drug-resistance regions in Mycobacterium tuberculosis using a long-read sequencing platform, followed by analysis with a tuberculosis-specific bioinformatics pipeline designed for long-read data.
10:11
Portable Intermodal Preferential Looking (IPL): Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism
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Cited by 32 •
2012
A reliable home-based way to assess the language comprehension of very young typically developing children, as well as those with autism, is described. The method analyzes children's eye gaze while viewing side-by-side images but hearing an audio that matches only one image. Stimuli are designed with young participants in mind.
07:33
Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Cited by 8 •
2017
Nanopore technology for sequencing biomolecules has wide applications in the life sciences, including identification of pathogens, food safety monitoring, genomic analysis, metagenomic environmental monitoring, and characterization of bacterial antibiotic resistance. In this article, the procedure for metagenomic soil DNA sequencing for species identification using the nanopore sequencing technology is demonstrated.
07:01
A New Portable In Vitro Exposure Cassette for Aerosol Sampling
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Cited by 4 •
2019
Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.
12:08
Field Identification of Matricaria chamomilla using a Portable qPCR System
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2020
Presented here is a protocol for field identification of Matricaria chamomilla using a portable qPCR system. This easy-to-perform protocol is ideal as a method to confirm the identity of a botanical species at locations where access to laboratory equipment and expertise is limited, such as farms and warehouses.
FAQs
Within the instrument, prepared nucleic acids move through nanoscale pores that produce changes in ionic current as bases pass through. Those electrical patterns provide the measurement stream, and software translates successive signal changes into sequence data. The key analytical challenge is linking current variation to base identity consistently enough for useful molecular characterization.
Sample preparation is critical because the instrument analyzes prepared nucleic acids rather than an unprocessed environmental sample. The quality and suitability of that preparation affect what enters the sequencing process, while sequencing accuracy and downstream interpretation influence how confidently results describe a habitat, community, or detected organism. These factors must be considered together.
Portability changes the timing and location of analysis rather than eliminating analytical requirements. Measurements can be made where environmental samples are collected, supporting near-real-time decisions and repeated monitoring. However, mobile operation still depends on adequate preparation, reliable signal-to-sequence conversion, and careful interpretation, so convenience does not remove the need for careful analytical practice.
A field workflow begins with collecting an environmental sample and preparing its nucleic acids for analysis. The prepared material is then introduced to the portable instrument, where pore-associated ionic-current changes are recorded. Software converts those signals into sequence data, which researchers interpret in relation to the environmental question, such as community composition or organism detection.
Environmental DNA analysis uses genetic material from water, soil, or another habitat to support biodiversity tracking. Sequence data can also help researchers investigate whether invasive species or pathogens are present. The value of each result depends on suitable preparation and interpretation, because molecular signals must be connected to the environmental context in which they were collected.
For microbial-community studies, portable sequencing can provide molecular information from environmental habitats without requiring every analysis to occur in a centralized laboratory. Applying the approach to water and soil supports characterization of their microbial communities, while repeated measurements can reveal changes over time. This makes the technique relevant to ongoing environmental monitoring.