Apc Fluorescence
Apc Fluorescence - Related Videos
05:21
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
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Cited by 1 •
2014
By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.
03:14
Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting
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2025
This video demonstrates the procedure for isolating and sorting nuclei from fresh-frozen human brain tissue using fluorescence-activated nuclei sorting (FANS), which enables the study of different cell populations for various neurological applications.
10:03
Quantification of Cytokine-Induced Cell Death in Human Colonic Organoids Using Live Fluorescence Microscopy
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2024
This protocol describes a simple and cost-effective method to investigate and quantify cell death in human colonic organoids in response to cytotoxic perturbagens such as cytokines. The approach employs a fluorescent cell death dye (SYTOX Green Nucleic Acid Stain), live fluorescence microscopy, and open-source image analysis software to quantify single-organoid responses to cytotoxic stimuli.
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
08:54
Bimolecular Fluorescence Complementation
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Cited by 66 •
2011
The subcellular localization of proteins is important in determining the spatio-temporal regulation of cell signaling. Here, we describe bimolecular fluorescence complementation (BiFC) as a straightforward method for monitoring the spatial interactions of proteins in the cell.
FAQs
After absorbing red excitation light, APC briefly reaches a higher-energy state and then releases part of that energy as red-to-far-red emission. Because the emitted light has a longer wavelength than the excitation light, an instrument can distinguish the fluorescent signal from the illumination source. This shift enables detection of APC-tagged antibodies and probes in measured samples.
APC provides bright emission in a red-to-far-red region that can complement fluorophores emitting at shorter wavelengths. This separation can reduce interference between signals, allowing several labeled targets to be assessed in the same sample. Its compatibility with multicolor panels makes it useful when researchers need to characterize multiple immune-cell markers or responses simultaneously.
Spectral spillover occurs when fluorescence from one label contributes to the signal recorded in another detection region. In panels containing APC and other fluorophores, this effect can complicate interpretation even when the targets differ biologically. Compensation helps account for the overlapping measured signals, improving separation of APC-associated fluorescence from neighboring signals.
APC-conjugated antibodies and other probes can support measurements of immune-cell populations, surface markers, cytokine responses, and pathogen-specific signals. The probe supplies fluorescence associated with its target, while flow cytometric analysis records the signal across cells or samples. This links molecular recognition to quantitative characterization of immune phenotypes and responses.
A researcher selects an APC-conjugated antibody or probe directed toward the marker or signal of interest, applies it to the sample, and analyzes the labeled material with an instrument capable of exciting APC and detecting its emission. The resulting fluorescence supports identification and measurement of relevant cell populations or responses, with compensation considered when signals overlap.
In immunology, APC labeling helps distinguish immune-cell populations and evaluate marker expression or cytokine responses. In infection studies, the same strategy can reveal pathogen-specific signals alongside cellular phenotypes. Combining these measurements in multicolor analyses allows researchers to relate immune-cell identity to functional or infection-associated responses within the same experimental framework.