Ex Vivo Gut
Ex Vivo Gut - Related Videos
04:09
An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions
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2025
In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue viability.
17:36
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
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Cited by 11 •
2010
We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 18 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
08:02
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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Cited by 1 •
2020
Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.
01:15
Assessing the Role of Gut Microbiota in Modulating Hepatitis B Infection in Mice
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2026
Take a control mouse with a normal gut microbiota and an experimental mouse with depleted gut microbiota. The depletion compromises the integrity of the gut barrier.This enables bacteria to translocate through the blood vessel into the liver tissue.Inject both mice with the hepatitis B virus or HBV plasmid to induce an HBV infection in the liver tissue.In the control mouse, the T cell detects an infected liver cell through the interaction of its receptors with viral antigens, and produces...
FAQs
Maintaining the tissue’s native architecture keeps epithelial cells, immune populations, and local barriers arranged in relationships that isolated cells may not reproduce. This organization allows investigators to connect molecular changes with tissue-level effects, such as barrier disruption or inflammatory signaling, and to observe how different intestinal components respond within the same experimental system.
These conditions support continued cellular function after tissue removal. Suitable media provide nutrients, while controlled oxygen and other environmental parameters help preserve epithelial, immune, and barrier activities long enough for manipulation and observation. If these requirements are not maintained, experimental findings may reflect loss of tissue function rather than the microbial, therapeutic, or inflammatory stimulus being studied.
Isolated cell cultures can clarify responses at the cellular or molecular level, but they do not retain the full tissue context. Ex vivo gut models preserve interactions among epithelial cells, immune populations, and local barriers, allowing host-pathogen effects to be examined alongside tissue responses. This makes the approach useful for linking mechanism to barrier disruption and inflammation.
The model supports investigation of inflammatory signaling, immune responses, and changes in intestinal barrier behavior after microbial or therapeutic stimulation. Because epithelial and immune components remain together, researchers can examine how local biology shapes host responses rather than analyzing each population in isolation. These observations help relate infection-associated signals to functional tissue outcomes.
A basic workflow involves placing intestinal tissue or organ segments in suitable culture media, supplying oxygen and nutrients, and maintaining controlled environmental conditions. Researchers then introduce the selected microbial, therapeutic, or other experimental stimulus, followed by observation of tissue responses. The setup must preserve function long enough to support meaningful manipulation and analysis.
Researchers can choose this approach when they need to study host-pathogen interactions or inflammatory responses in an intact tissue context without relying immediately on a whole-animal experiment. It is particularly informative for examining barrier disruption and responses to microbial or therapeutic stimuli, because the model retains relevant local biology while permitting controlled experimental manipulation.
Experiments can reveal tissue-level effects associated with barrier disruption, inflammatory signaling, host-pathogen interactions, or responses to microbial and therapeutic stimuli. The model complements cell cultures by adding tissue context and complements animal studies by focusing observation under controlled laboratory conditions. Its findings can therefore connect molecular mechanisms with functional changes in intestinal tissue.